川芎嗪通过丝裂原活化蛋白激酶通路在体外促进缺氧条件下大鼠脑源性神经干细胞的增殖和分化。
Tetramethylpyrazine promotes proliferation and differentiation of neural stem cells from rat brain in hypoxic condition via mitogen-activated protein kinases pathway in vitro.
机构信息
Institute of Neurobiology, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, The State Key Subject for Physiology, Xi'an Jiaotong University, College of Medicine, Xi'an, China.
出版信息
Neurosci Lett. 2010 Apr 19;474(1):26-31. doi: 10.1016/j.neulet.2010.02.066. Epub 2010 Mar 3.
This study investigated the effects of tetramethylpyrazine (TMP), an active element of traditional Chinese medicine Ligusticum Chuanxiong, on proliferation and differentiation of neural stem cells (NSCs) from rat brain in hypoxia condition and the activation of mitogen-activated protein kinases (MAPKs) signaling pathway during the processes. The results showed that TMP promoted the proliferation and differentiation of the NSCs into neurons. TMP increased the phosphorylation of ERK1/2 and decreased the phosphorylation of p38 at different time points. ERK inhibitor (U0126) in part blocked the differentiation of the NSCs into neurons induced by TMP. Our findings demonstrated that TMP enhanced the proliferation and differentiation of NSCs of rat after hypoxia in vitro, in which the phosphorylation of ERK and p38 was involved.
本研究探讨了川芎嗪（TMP）——一种中药川芎的有效成分——对缺氧条件下大鼠脑源性神经干细胞（NSCs）增殖和分化的影响，以及在这一过程中丝裂原活化蛋白激酶（MAPKs）信号通路的激活作用。结果表明，TMP 可促进 NSCs 向神经元的增殖和分化。TMP 在不同时间点增加 ERK1/2 的磷酸化，降低 p38 的磷酸化。ERK 抑制剂（U0126）部分阻断了 TMP 诱导的 NSCs 向神经元分化。我们的研究结果表明，TMP 可增强体外缺氧后大鼠 NSCs 的增殖和分化，其中 ERK 和 p38 的磷酸化参与其中。
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