利用三次谐波产生显微镜对秀丽隐杆线虫胚胎发生进行成像。

Imaging Caenorhabditis elegans embryogenesis by third-harmonic generation microscopy.

机构信息

Institute of Electronic Structure and Laser, Foundation of Research and Technology-Hellas, P.O. Box 1385, 71110, Heraklion, Crete, Greece.

出版信息

Micron. 2010 Jul;41(5):444-7. doi: 10.1016/j.micron.2010.02.006. Epub 2010 Feb 18.

DOI:10.1016/j.micron.2010.02.006

PMID:20207548

Abstract

In this study, third-harmonic generation (THG) imaging measurements were performed to characterize different developmental stages of the nematode Caenorhabditis elegans (C. elegans) embryos. Femtosecond laser pulses (1028 nm) were utilized for excitation. THG image contrast modality proved as a powerful diagnostic tool, providing valuable information and offering new insights into the complex developmental process of C. elegans embryogenesis.

摘要

在这项研究中，进行了三次谐波产生（THG）成像测量，以表征秀丽隐杆线虫（C. elegans）胚胎的不同发育阶段。飞秒激光脉冲（1028nm）用于激发。THG 图像对比模式被证明是一种强大的诊断工具，为秀丽隐杆线虫胚胎发生的复杂发育过程提供了有价值的信息和新的见解。