Balakrishna Asha Manikkoth, Hunke Cornelia, Grüber Gerhard
Nanyang Technological University, School of Biological Sciences, 60 Nanyang Drive, Singapore 637551, Singapore.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Mar 1;66(Pt 3):324-6. doi: 10.1107/S1744309110001016. Epub 2010 Feb 25.
A(1)A(o) ATP synthases are the major energy producers in archaea. Subunit E of the stator domain of the ATP synthase from Pyrococcus horikoshii OT3 was cloned, expressed and purified to homogeneity. The monodispersed protein was crystallized by vapour diffusion. A complete diffraction data set was collected to 3.3 A resolution with 99.4% completeness using a synchrotron-radiation source. The crystals belonged to space group I4, with unit-cell parameters a = 112.51, b = 112.51, c = 96.25 A, and contained three molecules in the asymmetric unit.
A(1)A(o)型ATP合酶是古生菌中的主要能量产生者。克隆、表达并纯化了来自嗜热栖热菌OT3的ATP合酶定子结构域的E亚基,使其达到均一状态。通过气相扩散法使单分散的蛋白质结晶。使用同步辐射源收集了一个完整的衍射数据集,分辨率达到3.3 Å,完整性为99.4%。晶体属于空间群I4,晶胞参数a = 112.51、b = 112.51、c = 96.25 Å,不对称单元中包含三个分子。