Nakamura Toshiyuki, Kitamoto Noritoshi, Osawa Toshihiko, Kato Yoji
Graduate School of Human Science and Environment, University of Hyogo, Himeji, Japan.
Biosci Biotechnol Biochem. 2010;74(3):536-40. doi: 10.1271/bbb.90728. Epub 2010 Mar 7.
In a previous study we prepared monoclonal antibody against allyl isothiocyanate (AITC)-modified lysine (Lys), and found that AITC reacted with Lys under physiological conditions in vitro (T. Nakamura et al., Chem. Res. Toxicol., 22, 536-542 (2009)). In the present study, antibodies against benzyl isothiocyanate (ITC), 6-methylsulfinylhexyl ITC and phenethyl ITC modified protein were prepared, and the respective monoclonal antibodies, B6C9, 6MS3D10, and PE3A10 were obtained. These antibodies were applied to ITC detection in food using shredded Wasabia japonica (wasabi) and ground Carica papaya (papaya) seed by trapping ITC with biotin-labeled bovine serum albumin. ITC formation from the wasabi and papaya seed samples was confirmed using the antibodies in a dose-dependent manner. These antibodies might be applicable in identifying food-derived ITC.
在之前的一项研究中，我们制备了抗烯丙基异硫氰酸酯（AITC）修饰赖氨酸（Lys）的单克隆抗体，并发现AITC在体外生理条件下可与Lys发生反应（T. Nakamura等人，《化学研究毒理学》，22，536 - 542（2009））。在本研究中，制备了抗苄基异硫氰酸酯（ITC）、6 - 甲基亚磺酰己基ITC和苯乙基ITC修饰蛋白的抗体，并分别获得了单克隆抗体B6C9、6MS3D10和PE3A10。通过用生物素标记的牛血清白蛋白捕获ITC，将这些抗体应用于用磨碎的山葵（山嵛菜）和磨碎的番木瓜（木瓜）种子检测食品中的ITC。使用这些抗体以剂量依赖的方式证实了山葵和木瓜种子样品中ITC的形成。这些抗体可能适用于鉴定食品来源的ITC。
