Michael Agnieszka, John Justin, Meyer Brendan, Pandha Hardev
Postgraduate Medical School-Oncology, University of Surrey, Manor Park, Guildford, UK.
ScientificWorldJournal. 2010 Mar 5;10:393-401. doi: 10.1100/tsw.2010.37.
Live attenuated bacterial vectors, such as Salmonella typhimurium, have shown promise as delivery vehicles for DNA. We have examined two new strains of S. typhimurium and their impact on dendritic cell maturation (CD12-sifA/aroC mutant and WT05-ssaV/aroC, both in TML background). Strain WT05 matured dendritic cells in a more efficient way; caused higher release of cytokines TNF-alpha, IL-12, IL-1beta; and was efficient for gene transfer. These findings suggest that the genetic background of the attenuation can influence the pattern of inflammatory immune response to Salmonella infection.
减毒活细菌载体，如鼠伤寒沙门氏菌，已显示出作为DNA递送载体的潜力。我们研究了两种新型鼠伤寒沙门氏菌菌株及其对树突状细胞成熟的影响（TML背景下的CD12-sifA/aroC突变体和WT05-ssaV/aroC）。WT05菌株能更有效地使树突状细胞成熟；导致细胞因子TNF-α、IL-12、IL-1β的释放量更高；并且在基因转移方面效率更高。这些发现表明，减毒的遗传背景可能会影响对沙门氏菌感染的炎性免疫反应模式。
