固相萃取-高效液相色谱法测定复方吴茱萸巴布剂中吴茱萸碱和吴茱萸次碱在血浆中的含量及其药代动力学研究
[Determination of evodiamine and rutaecarpine of compound Wuzhuyu cataplasm in plasma by SPE-HPLC: application to its pharmacokinetics].
作者信息
Yin Wenguang, Li Manling, Liu Shuzhi, Kang Chen, Wang Yanli, Zhou Zhongming, Tong Huijuan
机构信息
Institute of Chinese Meteria Maddica, China Academy of Chinese Medical Sciense, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(22):2946-9.
OBJECTIVE
To establish a SPE-HPLC method for the determination and pharmacokinetic study of evodiamine and rutacarpine in rat plasma.
METHOD
A Kromasil C18 column (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-water-tetrahydrofuran-acetic acid (51:48:1:0.1) as a mobile phase and at a flow rate of 1 mL x min(-1), and the UV detection was at 225 nm. The column temperature was 35 degrees C. After the analytes were extracted from the plasma of rats by solid phase extraction (SPE), the content of evodiamine and rutaecarpine was measuared by HPLC method using halcinonide as an internal standard solution.
RESULT
After transdermal administration to rats, the pharmacokinetic behavior of evodiamine and rutaecarpine belongs to the one-compartment model. The main pharmacokinetic parameters was as follows: K(a) 0.224 h(-1) and 0.220 h(-1), K(e) 0.114 h(-1) and 0.118 h(-1), C(max) 0.211 mg x L(-1) and 0.272 mg x L(-1), T(peak) 6.132 h and 6.102 h, respectively.
CONCLUSION
The method in this study is simple, rapid and sensitive. It is proved to be suitable for pharmacokinetic study of evodiamine and rutaecarpine.
目的
建立固相萃取-高效液相色谱法测定大鼠血浆中吴茱萸碱和吴茱萸次碱，并进行药代动力学研究。
方法
采用Kromasil C18柱（4.6 mm×250 mm，5μm），以乙腈-水-四氢呋喃-乙酸（51:48:1:0.1）为流动相，流速为1 mL·min⁻¹，紫外检测波长为225 nm，柱温为35℃。大鼠血浆中的分析物经固相萃取（SPE）提取后，以哈西奈德为内标溶液，采用高效液相色谱法测定吴茱萸碱和吴茱萸次碱的含量。
结果
大鼠经皮给药后，吴茱萸碱和吴茱萸次碱的药代动力学行为符合一室模型。主要药代动力学参数如下：K(a)分别为0.224 h⁻¹和0.22（此处原文有误，应为0.220）h⁻¹，K(e)分别为0.114 h⁻¹和0.118 h⁻¹，C(max)分别为0.211 mg·L⁻¹和0.272 mg·L⁻¹，T(peak)分别为6.132 h和6.102 h。
结论
本研究方法简便、快速、灵敏，适用于吴茱萸碱和吴茱萸次碱的药代动力学研究。
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