University of California Irvine, Beckman Laser Institute, Irvine, California 92612, USA. mail:
J Biomed Opt. 2010 Jan-Feb;15(1):017012. doi: 10.1117/1.3299322.
We present a novel, noncontact method for the determination of quantitative optical properties of turbid media from 430 to 1050 nm. Through measuring the broadband reflectance from an unknown sample as a function of the spatial frequency of the projected illumination patterns, the absolute absorption and reduced scattering coefficients can be calculated without a priori assumptions of the chromophores present. This technique, which is called spatially modulated quantitative spectroscopy (SMoQS), was validated through the quantification of optical properties of homogenous liquid phantoms with known concentrations of absorbers and scatterers. The properties of the phantoms were recovered across the range of values prepared with R(2) values of 0.985 and 0.996 for absorption and reduced scattering, respectively. A measurement was also performed on skin tissue as a demonstration of the method's performance in vivo. The resultant absorption spectrum was well described by a multichromophore fit, and the quantitative values for oxy- and deoxyhemoglobin, water, and melanin were within published ranges for skin.
我们提出了一种新颖的、非接触式方法，用于从 430 到 1050nm 范围内测定混浊介质的定量光学特性。通过测量未知样品对投影照明图案的空间频率的宽带反射率，可以在没有对存在的发色团进行先验假设的情况下计算绝对吸收和散射系数。这项名为空间调制定量光谱学（SMoQS）的技术已通过对具有已知吸收剂和散射剂浓度的同质液体模型的光学性质进行定量验证。通过对准备好的一系列具有 0.985 和 0.996 的 R(2)值的吸收和散射值，该模型在整个范围内的性能得到了恢复。还对皮肤组织进行了测量，以证明该方法在体内的性能。所得的吸收光谱很好地描述了多色团拟合，并且氧合和脱氧血红蛋白、水和黑色素的定量值在皮肤的发表范围内。
