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一种由 DNA 构成的多功能内切核酸酶模拟物:8-17 RNA 切割 DNA 酶的特性和应用。

A versatile endoribonuclease mimic made of DNA: characteristics and applications of the 8-17 RNA-cleaving DNAzyme.

机构信息

Department of Biochemistry and Biomedical Sciences and Department of Chemistry and Chemical Biology, McMaster University, 1200 Main St. W., Hamilton, Ontario, Canada.

出版信息

Chembiochem. 2010 May 3;11(7):866-79. doi: 10.1002/cbic.200900786.

Abstract

Enzymes play a crucial role in all living organisms by accelerating the rates of a myriad of biochemical reactions that are necessary to sustain life. Although the vast majority of known enzymes are made of protein, in recent years it has become increasingly apparent that other molecular formats, like nucleic acids, can also serve in this capacity. DNAzymes (also known as deoxyribozymes) are synthetic enzymes made of short, single strands of deoxyribonucleic acid. These DNA-based enzymes offer the prospect of significant commercial utility, because they are exceptionally stable and can be produced very easily and inexpensively. The study of one particular DNAzyme, known as "8-17", has enhanced our understanding of DNAzyme-mediated catalysis. Moreover, the function of 8-17 has been regarded with special importance because it can catalyze sequence-specific cleavage of RNA, a reaction that has broad implications in biotechnology and biomedical fields. In this review, we explore the creation, characterization, and application of the 8-17 RNA-cleaving DNAzyme.

摘要

酶在所有生物体中都起着至关重要的作用,通过加速无数种生物化学反应的速率来维持生命。尽管绝大多数已知的酶都是由蛋白质构成的,但近年来越来越明显的是,其他分子形式,如核酸,也可以起到这种作用。DNA 酶(也称为脱氧核酶)是由短的、单链的脱氧核糖核酸制成的合成酶。这些基于 DNA 的酶具有重要的商业应用前景,因为它们非常稳定,并且可以非常容易且廉价地生产。对一种被称为“8-17”的特定 DNA 酶的研究增强了我们对 DNA 酶介导的催化的理解。此外,8-17 的功能受到特别重视,因为它可以催化 RNA 的序列特异性切割,这一反应在生物技术和生物医学领域具有广泛的意义。在这篇综述中,我们探讨了 8-17 RNA 切割 DNA 酶的创建、表征和应用。

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