Li Hong-shan, Feng Qin, Hu Yi-yang
Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2009 Dec;29(12):1092-5.
To explore the intervention effect of Qushi Huayu Decoction (QHD) on high-fat diet induced hepatic lipid deposition and its dose-effect relationship in rats.
Fatty liver model of rats were established simply by 10 weeks of high-fat diet feeding, and starting from the 7th week of modeling, they were gastric perfused respectively with saline (model group), high-dose QHD (QHDh group), low-dose QHD (QHDI group) and polyene phosphatidylcholine (PP group) for successive 4 weeks. Liver pathology by electron microscope observation with HE staining and oil red staining; contents of triglyceride (TG) and free fatty acid (FFA) in liver tissue; and serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), and TG in rats were determined.
In the model group, the significant hepatic steatosis and vesicle changes as well as severe accumulation of middle- and micro-sized fatty drops in the hepatocyte plasma were found under electron microscope; with TG and FFA contents in liver tissue elevated to 3.2 and 3.5 multiples of those in normal group respectively, but, the difference between them in serum levels of ALT, AST, TG and TC were not significant. Above-mentioned pathological changes in the QHDh, QHDI and PP groups were all ameliorated significantly with the hepatic TG decreased to 57.55%, 72.32% and 71.07%, and FFA decreased to 48.95%, 65.67%, 55.57% of those in model group respectively, especially the effect of QHDh in reducing TG was superior to that of QHDI and PP (P < 0.05).
QHD shows an evident fatty liver antagonizing effect in rats induced by high-fat diet in a dose-dependent manner.
探讨祛湿化瘀方（QHD）对高脂饮食诱导的大鼠肝脏脂质沉积的干预作用及其量效关系。
通过10周高脂饮食喂养建立大鼠脂肪肝模型，从造模第7周开始，分别用生理盐水（模型组）、高剂量QHD（QHDh组）、低剂量QHD（QHDI组）和多烯磷脂酰胆碱（PP组）进行连续4周的胃灌注。采用HE染色和油红染色通过电子显微镜观察肝脏病理；测定肝组织中甘油三酯（TG）和游离脂肪酸（FFA）的含量；以及大鼠血清中丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、总胆固醇（TC）和TG的水平。
模型组电子显微镜下可见明显的肝脏脂肪变性和空泡改变，肝细胞质内有大量中、小脂肪滴严重堆积；肝组织中TG和FFA含量分别升高至正常组的3.2倍和3.5倍，但血清中ALT、AST、TG和TC水平差异无统计学意义。QHDh组、QHDI组和PP组上述病理改变均明显改善，肝组织TG分别降至模型组的57.55%、72.32%和71.07%，FFA分别降至模型组的48.95%、65.67%、55.57%，尤其QHDh组降低TG的效果优于QHDI组和PP组（P<0.05）。
QHD对高脂饮食诱导的大鼠脂肪肝具有明显的拮抗作用，且呈剂量依赖性。
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