Zhang Hui, Hu Yi-Yang, Feng Qin
Shuguang Hospital Affiliated to Shanghai University of TCM, Institute of Liver Disease of Shanghai University of TCM, Shanghai.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2007 Dec;27(12):1105-9.
To study the effects of Qushi Huayu Decoction (QHD) contained serum on fatty deposition and tumor necrosis factor alpha (TNF-alpha) secretion of hepatic lipotoxicity model in vitro, for further investigating the mechanism of the decoction for preventing and treating fatty liver.
The steatosis with TNF-alpha secretion lipotoxic model of HepG2 induced by long-chain free fatty acid (FFA) was duplicated. Groups of normal, model cells and model cells treated with different concentrations of QHD contained serum were set up to test the content of TNF-alpha in culture supernate and triglyceride (TG) in cells, as well as to observe the ultrastructural change of cells by oil-red staining and the protein expression and gene expression of cellular TNF-alpha.
After being stimulated with FFA for 24 h, marked deposition of lipid with high content of TG presented in the cells of model group. Compared with the normal group, not only TNF-alpha content of culture supernate but also the protein expression and mRNA expression of intracellular TNF-alpha increased significantly. Contrast to the model group, the contents of TG in cells and TNF-alpha in supernate as well as the protein and mRNA expression of TNF-alpha in the model cell group treated with 10% QHD were lower significantly (all P < 0.01).
QHD could significantly inhibit the fatty deposition and TNF-alpha secretion in HepG2 cells induced by free fatty acid.
研究祛湿化瘀方含药血清对体外肝脂毒性模型脂肪沉积及肿瘤坏死因子α（TNF-α）分泌的影响，进一步探讨该方防治脂肪肝的作用机制。
复制长链游离脂肪酸（FFA）诱导的HepG2脂肪变性伴TNF-α分泌的脂毒性模型。设立正常组、模型细胞组以及不同浓度祛湿化瘀方含药血清处理的模型细胞组，检测培养上清液中TNF-α含量、细胞内甘油三酯（TG）含量，通过油红染色观察细胞超微结构变化以及细胞TNF-α的蛋白表达和基因表达。
FFA刺激24 h后，模型组细胞出现明显的脂质沉积，TG含量升高。与正常组相比，培养上清液中TNF-α含量以及细胞内TNF-α的蛋白表达和mRNA表达均显著增加。与模型组相比，10%祛湿化瘀方含药血清处理的模型细胞组细胞内TG含量、上清液中TNF-α含量以及TNF-α的蛋白和mRNA表达均显著降低（均P<0.01）。
祛湿化瘀方能够显著抑制游离脂肪酸诱导的HepG2细胞脂肪沉积及TNF-α分泌。
