Humm K, Adamantos S
Department of Veterinary Clinical Sciences, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA.
J Small Anim Pract. 2010 Apr;51(4):200-3. doi: 10.1111/j.1748-5827.2009.00905.x. Epub 2010 Mar 1.
To describe a method of faecal smear production and to determine the sensitivity and specificity of faecal smear for detection of parasitic larvae using Baermann sedimentation as a gold standard.
Faecal smears were produced from samples submitted to the Royal Veterinary College Diagnostic Laboratory Service for Baermann sedimentation. An inexperienced and an experienced assessor each examined the smear for larvae for a maximum of five minutes.
One hundred and eighty six samples were analysed of which 28 were positive for Angiostrongylus vasorum on Baermann sedimentation. The experienced assessor had a faecal smear sensitivity of 61% and a specificity of 100%. The inexperienced assessor had a faecal smear sensitivity of 54% and a specificity of 95%.
Faecal smear is an effective and cheap aid to diagnosis of canine angiostrongylosis which can be readily performed in general practice. Its use leads to a rapid detection of infection compared to other available methods. This could lead to swifter treatment of the disease and a decrease in inappropriate diagnostics. A further diagnostic method, such as Baermann sedimentation, is recommended whether the faecal smear result is positive or negative.
描述粪便涂片制作方法,并以贝尔曼沉降法作为金标准,确定粪便涂片检测寄生虫幼虫的敏感性和特异性。
从提交给皇家兽医学院诊断实验室服务部进行贝尔曼沉降法检测的样本中制作粪便涂片。一名经验不足的评估人员和一名经验丰富的评估人员分别对涂片进行幼虫检查,最长检查五分钟。
共分析了186份样本,其中28份经贝尔曼沉降法检测显示血管圆线虫呈阳性。经验丰富的评估人员粪便涂片的敏感性为61%,特异性为100%。经验不足的评估人员粪便涂片的敏感性为54%,特异性为95%。
粪便涂片是诊断犬血管圆线虫病的一种有效且廉价的辅助手段,在一般临床实践中易于操作。与其他现有方法相比,使用该方法可快速检测出感染情况。这可能会使疾病得到更快治疗,并减少不恰当的诊断。无论粪便涂片结果是阳性还是阴性,都建议采用进一步的诊断方法,如贝尔曼沉降法。