Greer Laboratories Inc., 639 Nuway Circle, P.O. Box 800, Lenoir, NC 28645, United States.
J Immunol Methods. 2010 Apr 30;356(1-2):6-17. doi: 10.1016/j.jim.2010.03.004. Epub 2010 Mar 7.
The analysis of immunological markers in nasal secretions provides valuable information for studying nasal mucosa diseases and monitoring immunotherapy and immunity to vaccines administered locally. However, the concentration of biomarkers is highly variable in nasal secretions because of the diversity of collection methods. A nasal secretion collection device was developed to increase detectability of the assay, standardize the sampling technique, eliminate unknown dilution factor, and minimize analyte loss during the sample processing.
To develop and demonstrate the performance characteristics of a novel nasal secretion collector and its advantages over nasal lavage techniques.
Characteristics of absorption and recovery of the liquid or proteins by different types of polyurethane foam were evaluated. The concentration of immunoglobulins, inflammatory mediators and allergen specific antibodies was comparatively measured in nasal secretions collected by the novel nasal secretion collector and nasal lavages.
The concentrations of cytokines, eosinophil cationic protein, and tryptase in nasal secretions obtained by the nasal secretion collector were at least 8-fold higher than those tested in nasal lavages. Furthermore, the levels of immunoglobulins and the grass/weed pollen allergen specific antibodies were 6- to 290-fold increased when the nasal secretion collector was used. The nasal secretion collector was easy to use, non-invasive, and caused minimal discomfort to subjects during sampling.
A novel nasal secretion collector shows a significantly higher detectability and reproducibility than nasal lavages for analyzing immunological markers in nasal secretions.
The nasal secretion collector represented an approach for collecting nasal secretions and can be applied for routine evaluations of nasal immune responses induced by mucosal vaccinations or infections, inflammations and therapeutic interventions.
分析鼻腔分泌物中的免疫标志物可为研究鼻腔黏膜疾病以及监测局部免疫疗法和疫苗免疫提供有价值的信息。然而，由于收集方法的多样性，鼻腔分泌物中生物标志物的浓度变化很大。本研究开发了一种新型鼻腔分泌物采集装置，以提高检测的可检测性、标准化采样技术、消除未知稀释因子，并最大限度地减少样品处理过程中的分析物损失。
开发并展示新型鼻腔分泌物采集器的性能特点及其与鼻腔灌洗技术相比的优势。
评估不同类型的聚氨酯泡沫对液体或蛋白质的吸收和回收特性。比较了新型鼻腔分泌物采集器和鼻腔灌洗采集的鼻腔分泌物中免疫球蛋白、炎症介质和过敏原特异性抗体的浓度。
新型鼻腔分泌物采集器采集的鼻腔分泌物中细胞因子、嗜酸性粒细胞阳离子蛋白和类胰蛋白酶的浓度至少比鼻腔灌洗检测的浓度高 8 倍。此外，当使用鼻腔分泌物采集器时，免疫球蛋白和草/花粉过敏原特异性抗体的水平增加了 6 至 290 倍。新型鼻腔分泌物采集器易于使用、非侵入性，且在采样过程中对受试者造成的不适最小。
与鼻腔灌洗相比，新型鼻腔分泌物采集器在分析鼻腔分泌物中的免疫标志物方面具有更高的检测能力和重现性。
新型鼻腔分泌物采集器代表了一种采集鼻腔分泌物的方法，可用于常规评估黏膜疫苗接种或感染、炎症和治疗干预引起的鼻腔免疫反应。
