Bio-Rad Laboratories, Inc., Hercules, CA 94547, USA.
Methods. 2010 Apr;50(4):S1-5. doi: 10.1016/j.ymeth.2010.01.005.
Given the highly dynamic nature of mRNA transcription and the potential variables introduced in sample handling and in the downstream processing steps (Garson et al. (2009)), a standardized approach to each step of the RT-qPCR workflow is critical for reliable and reproducible results. The MIQE provides this approach with a checklist that contains 85 parameters to assure quality results that will meet the acceptance criteria of any journal (Bustin et al. (2009)). In this paper we demonstrate how to apply the MIQE guidelines (www.rdml.org/miqe) to establish a solid experimental approach.
鉴于 mRNA 转录的高度动态性质以及样品处理和下游处理步骤中引入的潜在变量（Garson 等人，2009 年），RT-qPCR 工作流程的每个步骤都需要标准化的方法，才能获得可靠且可重复的结果。MIQE 提供了这样一种方法，其中包含一个检查表，其中包含 85 个参数，可确保满足任何期刊的验收标准的高质量结果（Bustin 等人，2009 年）。在本文中，我们展示了如何应用 MIQE 指南（www.rdml.org/miqe）来建立可靠的实验方法。
