福尔马林固定石蜡包埋的人乳腺癌中PTEN表达的免疫组织化学检测方案。
Protocol for PTEN expression by immunohistochemistry in formalin-fixed paraffin-embedded human breast carcinoma.
作者信息
Sakr Rita A, Barbashina Violetta, Morrogh Mary, Chandarlapaty Sarat, Andrade Victor P, Arroyo Crispinita D, Olvera Narciso, King Tari A
机构信息
Sloan-Kettering Institute, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
出版信息
Appl Immunohistochem Mol Morphol. 2010 Jul;18(4):371-4. doi: 10.1097/PAI.0b013e3181d50bd5.
Abstract
The PI3K/PTEN pathway plays a major role in carcinogenesis. Dysregulation of this pathway occurs frequently in breast cancer, and loss of PTEN expression is emerging as a potentially important mechanism of resistance to the widely used anti-HER2 therapy, trastuzumab. However, assays for loss of PTEN expression have suffered from lack of consistency. Here, we describe an automated and reliable protocol for PTEN protein expression by immunohistochemistry in formalin-fixed paraffin-embedded tissue that can be easily incorporated into clinical trials.
摘要
PI3K/PTEN信号通路在肿瘤发生过程中起主要作用。该信号通路的失调在乳腺癌中频繁发生，PTEN表达缺失正成为对广泛使用的抗HER2疗法曲妥珠单抗产生耐药性的一个潜在重要机制。然而，PTEN表达缺失的检测方法一直缺乏一致性。在此，我们描述了一种通过免疫组织化学在福尔马林固定石蜡包埋组织中检测PTEN蛋白表达的自动化且可靠的方案，该方案可轻松纳入临床试验。
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2
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3
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