Kuźbicki Lukasz, Lange Dariusz, Stanek-Widera Agata, Chwirot Barbara W
Department of Medical Biology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Toruń, Poland.
Appl Immunohistochem Mol Morphol. 2010 Jul;18(4):357-64. doi: 10.1097/PAI.0b013e3181d4069c.
Lesions originating from different types of skin cells differ significantly with respect to their pathologic importance. The aim of this work was to examine as to what extent the differences in the origin are reflected in expression levels of CDK-2 and to investigate whether CDK-2 expression might be considered as potential marker useful for diagnostics and assessment of invasiveness of human nonmelanocytic lesions. We conducted comparative immunohistochemical studies of expression of cyclin-dependent kinase 2 (CDK-2) in 16 benign epithelial skin lesions, 11 precancerous lesions, 19 cases of basal cell carcinoma (first such study), 14 squamous cell carcinomas (SCCs), and 7 fibromas. Development of benign epithelial skin lesions was not associated with considerable increase of the CDK-2 expression. Increase of the CDK-2 level was observed in precancerous lesions, and the expression was strongest in SCCs. The level of CDK-2 may be related to invasiveness of skin cancers, as squamous cell carcinomas expressed the enzyme significantly stronger than basal cell carcinomas. Higher percentage fraction of CDK-2 positive cells observed in SCC compared with precancerous lesions may be useful for histopathologic diagnostics of this cancer. Moreover, strong immunohistochemical CDK-2 staining of the cancer cells present deep in dermis may facilitate their detection in histopathologic examinations.
源自不同类型皮肤细胞的病变在病理重要性方面存在显著差异。本研究的目的是探讨起源差异在细胞周期蛋白依赖性激酶2（CDK-2）表达水平上的体现程度，并研究CDK-2表达是否可被视为用于诊断和评估人类非黑素细胞性病变侵袭性的潜在标志物。我们对16例良性上皮性皮肤病变、11例癌前病变、19例基底细胞癌（首次此类研究）、14例鳞状细胞癌（SCC）和7例纤维瘤进行了细胞周期蛋白依赖性激酶2（CDK-2）表达的比较免疫组化研究。良性上皮性皮肤病变的发生与CDK-2表达的显著增加无关。在癌前病变中观察到CDK-2水平升高，且在鳞状细胞癌中表达最强。CDK-2水平可能与皮肤癌的侵袭性有关，因为鳞状细胞癌中该酶的表达明显强于基底细胞癌。与癌前病变相比，在鳞状细胞癌中观察到的CDK-2阳性细胞的更高百分比可能有助于该癌症的组织病理学诊断。此外，真皮深层存在的癌细胞的强烈免疫组化CDK-2染色可能有助于在组织病理学检查中对其进行检测。
