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小麦胚起始因子4F和（异）4F与兔α-珠蛋白mRNA的寡核糖核苷酸类似物的相互作用不同。

Wheat germ initiation factors 4F and (iso)4F interact differently with oligoribonucleotide analogues of rabbit alpha-globin mRNA.

作者信息

Carberry S E, Goss D J

机构信息

Department of Chemistry, Hunter College, City University of New York, New York 10021.

出版信息

Biochemistry. 1991 May 7;30(18):4542-5. doi: 10.1021/bi00232a025.

DOI:10.1021/bi00232a025
PMID:2021646
Abstract

The binding of capped oligoribonucleotide analogues of the 5' terminus of rabbit alpha-globin mRNA to wheat germ protein synthesis initiation factors eIF-4F and eIF-(iso)4F was measured by direct fluorescence techniques. An analysis of the equilibrium association constants (Keq) indicates that both eIF-4F and eIF-(iso)4F recognize primarily the m7G cap structure but differ in the recognition of other structural features. eIF-4F is sensitive to the position and sequence of hairpin structures within the oligoribonucleotide, while eIF-(iso)4F shows a preference for linear sequences. These differences suggest that wheat germ eIF-4F and eIF-(iso)4F may have discriminatory activity for mRNA recognition.

摘要

通过直接荧光技术测定了兔α-珠蛋白mRNA 5'末端的带帽寡核糖核苷酸类似物与小麦胚芽蛋白合成起始因子eIF-4F和eIF-(iso)4F的结合。对平衡缔合常数(Keq)的分析表明，eIF-4F和eIF-(iso)4F都主要识别m7G帽结构，但在识别其他结构特征方面存在差异。eIF-4F对寡核糖核苷酸内发夹结构的位置和序列敏感，而eIF-(iso)4F则偏好线性序列。这些差异表明，小麦胚芽eIF-4F和eIF-(iso)4F可能对mRNA识别具有鉴别活性。

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