Department of Anatomy, Showa University School of Medicine, Shinagawa-Ku, Tokyo 142-8555, Japan.
J Clin Biochem Nutr. 2010 Mar;46(2):93-104. doi: 10.3164/jcbn.09-61. Epub 2010 Feb 24.
Because rheumatoid arthritis (RA), an autoimmune disease, the patients often recognize side-effects due to the medication, alternative therapeutic strategies might potentially offer a clinical advantage. We evaluated the effect of nucleoprotein from salmon soft roe on animal model of arthritis. Mice transgenic for human T-cell leukemia virus type I (HTLV-1 Tg) were divided into three experimental groups and supplemented on either nucleoprotein-free (nonNP), or 0.6% or 1.2% nucleoprotein mixed (NP0.6 or NP1.2) diet for 3 months. The mice were evaluated arthritis by morphology, and measured with rheumatoid factor (RF). Moreover, macrophages and oxidative metabolites were assessed in the ankle and/or serum. Anti-oxidative potentials in nucleoprotein were determined with biological anti-oxidative potential (BAP) test, and electron spin resonance (ESR) analysis. NonNP-diet HTLV-1 Tg mice increased an arthritis symptoms and RF. The symptoms were ameliorated in NP-diet groups. Macrophages detected by F4/80 staining, and oxidative metabolites in the serum and/or joints were clearly decreased in 1.2% NP-diet HTLV-1 Tg mice. Nucleoprotein and DNA-nucleotide, but less protamine, had direct anti-oxidative potency with BAP test and/or ESR in vitro. These observations suggest that dietary nucleoprotein ameliorates arthritis symptoms in HTLV-1 Tg mice and offers hope as an alternative treatment for this debilitating medical condition.
由于类风湿性关节炎（RA）是一种自身免疫性疾病，患者经常因药物治疗而出现不良反应，因此替代治疗策略可能具有临床优势。我们评估了鲑鱼软鱼子核蛋白对关节炎动物模型的影响。人 T 细胞白血病病毒 I 型（HTLV-1 Tg）转基因小鼠分为三组，分别补充不含核蛋白（非 NP）或 0.6%或 1.2%核蛋白混合（NP0.6 或 NP1.2）饮食 3 个月。通过形态学评估关节炎，并通过类风湿因子（RF）进行测量。此外，在踝关节和/或血清中评估巨噬细胞和氧化代谢物。采用生物抗氧化潜力（BAP）试验和电子自旋共振（ESR）分析测定核蛋白中的抗氧化潜力。非 NP 饮食 HTLV-1 Tg 小鼠关节炎症状和 RF 增加。NP 饮食组的症状得到改善。通过 F4/80 染色检测到的巨噬细胞和血清和/或关节中的氧化代谢物在 1.2% NP 饮食 HTLV-1 Tg 小鼠中明显减少。核蛋白和 DNA-核苷酸具有直接的抗氧化能力，BAP 试验和/或 ESR 试验显示，鱼精蛋白的抗氧化能力较弱。这些观察结果表明，饮食核蛋白可改善 HTLV-1 Tg 小鼠的关节炎症状，并为这种使人衰弱的疾病提供了一种替代治疗方法。
