Botanical Raw Materials Division, Tsumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, Japan.
J Nat Med. 2010 Jul;64(3):257-65. doi: 10.1007/s11418-010-0397-6. Epub 2010 Mar 9.
Boi and its original plant Sinomenium acutum from Japan were compared with Seifuto and its botanical origins from China in terms of their internal transcribed spacer (ITS) sequences and major chemical components. Boi, Seifuto, and their botanical origins overall showed seven variable sites in the ITS sequence and six genotypes. Japanese S. acutum and Boi had one nucleotide variation at position 593 to show two genotypes (J1 and J2) and their heterozygote (J3). Seifuto samples and their botanical origins, S. acutum and S. acutum var. cinereum from China, showed three genotypes (C1, C2, and C3), which did not agree with the botanical classification, indicating that they cannot be distinguished according to their ITS sequences. All Seifuto samples from Henan market showed the same ITS genotype (C1). The Japanese and Chinese genotypes differed in the nucleotide position 424, which can be used to distinguish the country of origin of these materials. In the HPLC analysis of six major components, sinomenine (1), magnoflorine (2), menisperine (3), 6-O-methyllaudanosoline glucoside (4), liriodendrin (5), and menisdaurin (6), all were detected in Boi, whereas five (all except for menisdaurin) were detected in Seifuto. The main component in the rhizome of Seifuto was sinomenine, whereas magnoflorine was the main component in the rhizome and the climbing stem of Boi. The content of sinomenine in Seifuto was almost twice that in Boi. Although the individual content of alkaloids 1-4 differed between Boi and Seifuto, the total contents of these alkaloids were comparable between them both in the climbing stem and rhizome.
博落回及其源自日本的原植物青风藤与塞弗托及其源自中国的植物来源在其内部转录间隔区 (ITS) 序列和主要化学成分方面进行了比较。博落回、塞弗托及其植物来源在 ITS 序列中总体显示出 7 个变异位点和 6 个基因型。日本青风藤和博落回在位置 593 处有一个核苷酸变异，显示出两个基因型 (J1 和 J2) 和它们的杂合体 (J3)。塞弗托样品及其植物来源，来自中国的青风藤和青风藤 var. cinereum，显示出 3 个基因型 (C1、C2 和 C3)，这与植物分类不一致，表明不能根据 ITS 序列对它们进行区分。来自河南市场的所有塞弗托样品均显示出相同的 ITS 基因型 (C1)。日本和中国的基因型在核苷酸位置 424 上有所不同，可用于区分这些材料的原产国。在对 6 种主要成分的 HPLC 分析中，均检测到博落回中的盐酸青藤碱 (1)、粉防己碱 (2)、汉防己甲素 (3)、6-O-甲基黄连碱葡萄糖苷 (4)、蝙蝠葛苏林碱 (5)和蝙蝠葛任碱 (6)，而塞弗托中仅检测到 5 种成分 (均不含蝙蝠葛任碱)。塞弗托根茎的主要成分是盐酸青藤碱，而博落回根茎和攀援茎的主要成分是粉防己碱。塞弗托中盐酸青藤碱的含量几乎是博落回的两倍。尽管博落回和塞弗托中生物碱 1-4 的个体含量不同，但它们在攀援茎和根茎中的总含量相当。
