Department of Anesthesiology, Keio University, Tokyo, Japan.
Adv Exp Med Biol. 2010;669:177-80. doi: 10.1007/978-1-4419-5692-7_35.
Laudanosine, a degradation of neuomuscular blocking agent atracurium, crosses the blood-brain barrier and is indicted to trigger seizures at high concentration. In Xenopus Oocytes expressing nicotinic acetylcholine receptors (nAChRs), laudanosine has activating and inhibiting effects on nAChRs depending on its concentration. nAChRs is related to respiratory activities and thus, in the present study, we analyzed effects of laudanosine on central respiratory activities using isolated brainstem-spinal cord preparation of neonatal rats. The rhythmic inspiratory burst activity of the C4 spinal ventral root was recorded using a glass suction electrode as an index of respiratory rate. After superfusion with mock cerebrospinal fluid (CSF), the preparation was superfused with mock CSF containing laudanosine 1, 10 or 100 microM for 60 minutes. Laudanosine 1, 10 and 100 microM (n = 10 in each) did not induce any effects on C4 respiratory rate. In all 10 preparations, laudnosine 100 microM induced non-respiratory excitement activities that are possibly same as seizure observed in vivo study.
劳丹素是去极化型神经肌肉阻滞剂阿曲库铵的降解产物，它可以穿透血脑屏障，并在高浓度下引发癫痫发作。在表达烟碱型乙酰胆碱受体 (nAChRs) 的非洲爪蟾卵母细胞中，劳丹素的浓度依赖性激活或抑制 nAChRs。nAChRs 与呼吸活动有关，因此，在本研究中，我们使用新生大鼠离体延髓-脊髓标本分析了劳丹素对中枢呼吸活动的影响。使用玻璃吸管电极记录 C4 脊髓腹根的节律性吸气爆发活动作为呼吸频率的指标。在模拟脑脊液（CSF）灌流后，将标本用含劳丹素 1、10 或 100μM 的模拟 CSF 灌流 60 分钟。劳丹素 1、10 和 100μM（每组 10 个标本）对 C4 呼吸频率没有任何影响。在所有 10 个标本中，劳丹素 100μM 诱导出非呼吸兴奋活动，可能与体内研究中观察到的癫痫发作相同。
