Muhonen Pirkko, Holthofer Harry
Centre for BioAnalytical Sciences, Dublin City University, Dublin, Ireland.
Methods Mol Biol. 2010;623:93-107. doi: 10.1007/978-1-60761-588-0_6.
RNA interference mediated by short interfering RNA (siRNA) molecules represents a powerful genetic tool with an increasing interest as potential therapeutics. Current bioinformatic approaches to design functional siRNA molecules take into account both empirical and rational approaches to identify selectable characteristics of active and specific siRNA molecules and focusing the downstream events in the RNAi pathway, such as target messenger RNA accessibility. The design of effective siRNA molecules is the key to successful experimentation with RNAi. Here, we show advanced siRNA design parameters and options for highly efficient siRNA candidate search.
由短干扰RNA（siRNA）分子介导的RNA干扰是一种强大的遗传工具，作为潜在的治疗方法越来越受到关注。当前设计功能性siRNA分子的生物信息学方法既考虑了经验性方法，也考虑了理性方法，以识别活性和特异性siRNA分子的可选择特征，并聚焦RNAi途径中的下游事件，如靶信使RNA的可及性。设计有效的siRNA分子是RNAi成功实验的关键。在这里，我们展示了用于高效siRNA候选搜索的先进siRNA设计参数和选项。
