在多发性骨髓瘤细胞系中进行小干扰RNA（siRNA）转染。

Transfection of siRNAs in multiple myeloma cell lines.

作者信息

Brito Jose L R, Brown Nicola, Morgan Gareth J

机构信息

Section of Haemato-Oncology, Institute of Cancer Research, London, UK.

出版信息

Methods Mol Biol. 2010;623:299-309. doi: 10.1007/978-1-60761-588-0_19.

DOI:10.1007/978-1-60761-588-0_19

PMID:20217559

Abstract

RNA interference (RNAi), a valuable tool to specifically silence gene expression, is becoming an indispensable weapon in the arsenal of functional genomics, target validation and gene-specific therapeutic research. We have shown that Streptolysin-O (SLO) reversible permeabilization is an efficient method to deliver small interfering RNAs (siRNAs) to hard-to-transfect human myeloma cell lines. In addition, we have shown that transfection of siRNAs, using the SLO reversible permeabilization, specifically and efficiently induces gene silencing in myeloma cell lines.

摘要

RNA干扰（RNAi）是一种特异性沉默基因表达的重要工具，正成为功能基因组学、靶点验证和基因特异性治疗研究武器库中不可或缺的武器。我们已经证明，链球菌溶血素-O（SLO）可逆通透化是一种将小干扰RNA（siRNA）递送至难以转染的人骨髓瘤细胞系的有效方法。此外，我们已经证明，利用SLO可逆通透化转染siRNA可在骨髓瘤细胞系中特异性且高效地诱导基因沉默。