一种用于六组氨酸标签蛋白共价固定以进行表面等离子体共振的捕获偶联方法。
A capture coupling method for the covalent immobilization of hexahistidine tagged proteins for surface plasmon resonance.
作者信息
Kimple Adam J, Muller Robin E, Siderovski David P, Willard Francis S
机构信息
Department of Pharmacology, The University of North Carolina, Chapel Hill, NC, USA.
出版信息
Methods Mol Biol. 2010;627:91-100. doi: 10.1007/978-1-60761-670-2_5.
Abstract
Surface plasmon resonance (SPR) is a robust method to detect and quantify macromolecular interactions; however, to measure binding interactions, one component must be immobilized on a sensor surface. This is typically achieved using covalent immobilization via free amines or thiols, or noncovalent immobilization using high-affinity interactions such as biotin/streptavidin or antibody/antigen. In this chapter we describe a robust method to covalently immobilize His(6) fusion proteins on the sensor surface for SPR analysis.
摘要
表面等离子体共振（SPR）是一种用于检测和定量大分子相互作用的可靠方法；然而，为了测量结合相互作用，必须将一种成分固定在传感器表面。这通常通过经由游离胺或硫醇的共价固定，或使用诸如生物素/链霉亲和素或抗体/抗原等高亲和力相互作用的非共价固定来实现。在本章中，我们描述了一种将His(6)融合蛋白共价固定在传感器表面以进行SPR分析的可靠方法。
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