通过表面等离子体共振技术确定嗜热栖热放线菌ATCC 12980的S层蛋白SbsC与次生细胞壁聚合物之间的高亲和力相互作用。
High-affinity interaction between the S-layer protein SbsC and the secondary cell wall polymer of Geobacillus stearothermophilus ATCC 12980 determined by surface plasmon resonance technology.
作者信息
Ferner-Ortner Judith, Mader Christoph, Ilk Nicola, Sleytr Uwe B, Egelseer Eva M
机构信息
Center for NanoBiotechnology, University of Natural Resources and Applied Life Sciences, Gregor Mendel-Strasse 33, A-1180 Vienna, Austria.
出版信息
J Bacteriol. 2007 Oct;189(19):7154-8. doi: 10.1128/JB.00294-07. Epub 2007 Jul 20.
Abstract
Surface plasmon resonance studies using C-terminal truncation forms of the S-layer protein SbsC (recombinant SbsC consisting of amino acids 31 to 270 [rSbsC(31-270)] and rSbsC(31-443)) and the secondary cell wall polymer (SCWP) isolated from Geobacillus stearothermophilus ATCC 12980 confirmed the exclusive responsibility of the N-terminal region comprising amino acids 31 to 270 for SCWP binding. Quantitative analyses indicated binding behavior demonstrating low, medium, and high affinities.
摘要
使用嗜热栖热放线菌ATCC 12980的S层蛋白SbsC的C端截短形式（由氨基酸31至270组成的重组SbsC[rSbsC(31 - 270)]和rSbsC(31 - 443)）以及从嗜热栖热放线菌中分离出的次生细胞壁聚合物（SCWP）进行的表面等离子体共振研究证实，包含氨基酸31至270的N端区域对SCWP结合具有唯一责任。定量分析表明其结合行为表现出低、中、高亲和力。
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