表面等离子体共振/质谱联用技术：从传感芯片中回收用于基质辅助激光解吸电离飞行时间质谱法进行蛋白质鉴定的物质。
SPR/MS: recovery from sensorchips for protein identification by MALDI-TOF mass spectrometry.
作者信息
Borch Jonas, Roepstorff Peter
机构信息
Institute for Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.
出版信息
Methods Mol Biol. 2010;627:269-81. doi: 10.1007/978-1-60761-670-2_19.
Surface plasmon resonance is widely used to study binding interactions with proteins, potentially yielding information on kinetics, thermodynamics and active concentrations. However, the technology cannot identify the involved interaction partners. Mass spectrometry, on the other hand, can be used for specific identification of proteins in amounts comparable to the levels that can be captured on a Biacore SPR sensorchip. Here we present protocols for capturing, washing and eluting proteins from Biacore instruments as well as for robust sample preparation for sensitive mass spectrometric identification.
表面等离子体共振被广泛用于研究与蛋白质的结合相互作用，有可能产生关于动力学、热力学和活性浓度的信息。然而，该技术无法识别所涉及的相互作用伙伴。另一方面，质谱可用于以与Biacore SPR传感器芯片上可捕获的水平相当的量对蛋白质进行特异性鉴定。本文介绍了从Biacore仪器捕获、洗涤和洗脱蛋白质的方案，以及用于灵敏质谱鉴定的可靠样品制备方法。
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