Nedelkov D, Nelson R W
Intrinsic Bioprobes Inc., Tempe, AZ 85281, USA.
J Mol Recognit. 2000 May-Jun;13(3):140-5. doi: 10.1002/1099-1352(200005/06)13:3<140::AID-JMR496>3.0.CO;2-P.
Biomolecular interaction analysis mass spectrometry (BIA/MS) is a multiplexed analytical technique that utilizes a unique combination of surface plasmon resonance (SPR) and matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the detection and analysis of small amounts of proteins residing in complex biological systems. In order to achieve high sensitivity during BIA/MS, certain experimental parameters and sequences of events need to be optimized and maintained. Immobilized ligand density, flow rate and biosensor control (in SPR-BIA) and matrix choice and application (in MALDI-TOF MS) have significant influence on the final outcome of the BIA/MS analysis and, consequently, need to be optimized and carefully controlled. In addition, chip washing and cutting are essential in converting the SPR-active sensor chips into target surfaces amenable to MALDI-TOF MS. Reviewed here are the prerequisites for successfully interfacing SPR-BIA with MALDI-TOF MS.
生物分子相互作用分析质谱法（BIA/MS）是一种多重分析技术，它利用表面等离子体共振（SPR）和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）的独特组合，来检测和分析复杂生物系统中存在的少量蛋白质。为了在BIA/MS过程中实现高灵敏度，需要优化和维持某些实验参数及事件顺序。固定配体密度、流速和生物传感器控制（在SPR-BIA中）以及基质选择和应用（在MALDI-TOF MS中）对BIA/MS分析的最终结果有重大影响，因此需要进行优化并仔细控制。此外，芯片清洗和切割对于将SPR活性传感器芯片转化为适合MALDI-TOF MS的目标表面至关重要。本文综述了成功将SPR-BIA与MALDI-TOF MS联用的前提条件。
