Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA.
J Pathol. 2010 May;221(1):106-16. doi: 10.1002/path.2692.
Anaplastic plasmacytomas (APCTs) from NFS.V(+) congenic mice and pristane-induced plasmacytic PCTs from BALB/c mice were previously shown to be histologically and molecularly distinct subsets of plasma cell neoplasms (PCNs). Here we extended these comparisons, contrasting primary APCTs and PCTs by gene expression profiling in relation to the expression profiles of normal naïve, germinal centre, and memory B cells and plasma cells. We also sequenced immunoglobulin genes from APCT and APCT-derived cell lines and defined surface phenotypes and chromosomal features of the cell lines by flow cytometry and by spectral karyotyping and fluorescence in situ hybridization. The results indicate that APCTs share many features with normal memory cells and the plasma cell-related neoplasms (PLs) of FASL-deficient mice, suggesting that APCTs and PLs are related and that both derive from memory B cells. Published in 2010 by John Wiley & Sons, Ltd.
先前研究表明，NFS.V(+)同基因小鼠的间变性浆细胞瘤 (APCT) 和 pristane 诱导的浆细胞瘤是浆细胞肿瘤 (PCN) 的组织学和分子学上明显不同的亚群。在这里，我们通过基因表达谱分析，将原发性 APCT 和 PCT 与正常幼稚 B 细胞、生发中心 B 细胞和记忆 B 细胞及浆细胞的表达谱进行对比，进一步扩展了这些比较。我们还对来自 APCT 和 APCT 衍生细胞系的免疫球蛋白基因进行了测序，并通过流式细胞术和光谱核型分析及荧光原位杂交技术对细胞系的表面表型和染色体特征进行了定义。结果表明，APCT 与正常记忆细胞和 FasL 缺陷型小鼠的浆细胞相关肿瘤 (PL) 有许多共同特征，提示 APCT 和 PL 是相关的，并且均来源于记忆 B 细胞。该研究由 John Wiley & Sons, Ltd. 于 2010 年出版。
