从微阵列到基因组规模测序分析 DNA 甲基化组。
Profiling DNA methylomes from microarray to genome-scale sequencing.
机构信息
The Ohio State University Comprehensive Cancer Center, Columbus, OH 43210, USA.
出版信息
Technol Cancer Res Treat. 2010 Apr;9(2):139-47. doi: 10.1177/153303461000900203.
Abstract
DNA cytosine methylation is a central epigenetic modification which plays critical roles in cellular processes including genome regulation, development and disease. Here, we review current and emerging microarray and next-generation sequencing based technologies that enhance our knowledge of DNA methylation profiling. Each methodology has limitations and their unique applications, and combinations of several modalities may help build the entire methylome. With advances on next-generation sequencing technologies, it is now possible to globally map the DNA cytosine methylation at single-base resolution, providing new insights into the regulation and dynamics of DNA methylation in genomes.
摘要
DNA 胞嘧啶甲基化是一种重要的表观遗传修饰，在包括基因组调控、发育和疾病在内的细胞过程中发挥着关键作用。在这里，我们综述了当前和新兴的基于微阵列和下一代测序的技术，这些技术增强了我们对 DNA 甲基化谱分析的认识。每种方法都有其局限性和独特的应用，几种模式的组合可能有助于构建整个甲基组。随着下一代测序技术的进步，现在可以以单碱基分辨率全局绘制 DNA 胞嘧啶甲基化图谱，为研究基因组中 DNA 甲基化的调控和动态提供新的见解。
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