Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen, Hungary.
DNA Cell Biol. 2010 May;29(5):249-59. doi: 10.1089/dna.2009.0980.
Time-lapse video microscopy was designed to follow the movement of single cells for an unlimited period of time under physiological conditions. The system is based on two inverted microscopes located in a CO(2) incubator and equipped with charge-coupled device cameras connected to the computer. Frames were recorded every minute and the subsequent video sequence was converted to database form. The system was applied to describe the movements of normal HaCaT cells and Pb-treated cells causing the so-called apoptotic dance during cell death. The apoptotic movement was also followed in high-osmolarity glycerol-type mitogen-activated protein kinase (MAPK) null mutant of Fusarium proliferatum, a filamentous fungus, during osmotic stress. The shortest (20 min) and most vigorous death movements were observed in apoptotic fungal cells subjected to salt stress. The necrotic process at higher Pb concentration (50 microM) took 2-3 h, whereas the apoptotic process at lower Pb concentrations lasted from minutes to days.
延时视频显微镜旨在在生理条件下无限期地跟踪单个细胞的运动。该系统基于位于 CO(2)培养箱中的两台倒置显微镜，配备连接到计算机的电荷耦合器件 (CCD) 相机。每 1 分钟记录一帧，随后的视频序列转换为数据库形式。该系统用于描述正常 HaCaT 细胞和 Pb 处理的细胞的运动，导致细胞死亡过程中的所谓凋亡舞蹈。在渗透压应激下，丝状真菌 Fusarium proliferatum 的高渗甘油型丝裂原活化蛋白激酶 (MAPK) 缺失突变体中也跟踪了凋亡运动。在盐胁迫下，观察到最短（20 分钟）和最剧烈的死亡运动的凋亡真菌细胞。在较高 Pb 浓度（50 μM）下，坏死过程持续 2-3 小时，而在较低 Pb 浓度下，凋亡过程持续数分钟至数天。
