Department of Physiology, Fukuoka University School of Medicine, Fukuoka, Japan.
Cell Biol Int. 2010 Apr 27;34(6):615-20. doi: 10.1042/CBI20100102.
Adult BMSCs (bone marrow stromal cells) contain MSCs (mesenchymal stem cells). MSCs can differentiate into osteoblasts, chondrocytes, adipocytes and myoblasts and are thus considered useful in tissue engineering for therapeutic and clinical purposes. FCS (fetal calf serum) is usually included in the differentiation medium, but the clinical application of FCS may pose a problem for some patients. To improve the efficiency and safety of BMSC cultivation, the effect of SF (serum-free) conditions on the osteogenic differentiation of rat BMSCs was examined. In the presence of 10% FCS and osteogenic supplements, the cells formed mineralized von Kossa-positive deposits. Under SF conditions with osteogenic supplementation, however, the cells possessed cytosolic lipid vacuoles that could be stained with Oil Red O. The mRNA expression of PPARgamma (peroxisome proliferator-activated receptor gamma), an adipogenic marker, increased under the SF condition with osteogenic supplements. These data indicate that rat BMSCs differentiate into adipocytes under SF conditions even with osteogenic supplementation and that FCS is needed to induce proper osteogenic differentiation in rat BMSCs.
成体骨髓基质细胞（BMSCs）中包含间充质干细胞（MSCs）。MSCs 可分化为成骨细胞、软骨细胞、脂肪细胞和肌细胞，因此被认为在组织工程中具有治疗和临床应用价值。FCS（胎牛血清）通常包含在分化培养基中，但 FCS 的临床应用可能对某些患者构成问题。为了提高 BMSC 培养的效率和安全性，研究了 SF（无血清）条件对大鼠 BMSCs 成骨分化的影响。在存在 10％FCS 和成骨补充剂的情况下，细胞形成矿化 von Kossa 阳性沉积物。然而，在含有成骨补充剂的 SF 条件下，细胞具有细胞质脂滴，可以用油红 O 染色。成脂标志物过氧化物酶体增殖物激活受体γ（PPARγ）的 mRNA 表达在含有成骨补充剂的 SF 条件下增加。这些数据表明，大鼠 BMSCs 在 SF 条件下即使有成骨补充剂也会分化为脂肪细胞，并且 FCS 是诱导大鼠 BMSCs 适当成骨分化所必需的。
