白细胞介素-17 和白细胞介素-23 在 ALS 患者的血清和脑脊液中升高：反映了 Th17 细胞的激活？

Interleukin-17 and interleukin-23 are elevated in serum and cerebrospinal fluid of patients with ALS: a reflection of Th17 cells activation?

机构信息

Department of Neurology, Aeginition Hospital, Athens National University, School of Medicine, Athens, Greece.

出版信息

Acta Neurol Scand. 2010 Dec;122(6):425-9. doi: 10.1111/j.1600-0404.2010.01333.x.

DOI:10.1111/j.1600-0404.2010.01333.x

PMID:20219021

Abstract

BACKGROUND

There is evidence that immunological factors may involved in pathogenetic mechanisms of amyotrophic lateral sclerosis (ALS). Th17 cells are characterized by predominant production of IL-17 and are suggested to be crucial in destructive autoimmunity. Interleukin-23 (IL-23) appears to play a supporting role in the continued stimulation and survival of Th17.

PATIENTS AND METHODS

We measured by enzyme-like immunosorbent assay (ELISA) serum and cerebrospinal fluid (CSF) levels of IL-17 and IL-23 in 22 patients with ALS and 19 patients with other non-inflammatory neurological disorders (NIND) studied as a control group. IL-17 and IL-23 serum and CSF levels were also correlated with duration of the disease, the disability level and the clinical subtype of the disease onset in patients with ALS.

RESULTS

IL-17 and IL-23 serum levels were higher in patients with ALS as compared with patients with NIND (P = 0.015 and P = 0.002 respectively). IL-17 and IL-23 CSF levels were also increased in patients with ALS (P = 0.0006 and P = 0.000001 respectively). IL-17 and IL-23 levels were not correlated with disease duration, disability scale or clinical subtype of the disease onset in ALS patients.

CONCLUSIONS

Our findings suggest that these molecules may be involved in the pathogenetic mechanisms acting as potential markers of Th17 cells activation in ALS.

摘要

背景

有证据表明，免疫因素可能参与肌萎缩侧索硬化症（ALS）的发病机制。Th17 细胞的特征是主要产生 IL-17，并被认为在破坏性自身免疫中至关重要。白细胞介素-23（IL-23）似乎在 Th17 的持续刺激和存活中发挥支持作用。

患者和方法

我们通过酶联免疫吸附试验（ELISA）测量了 22 例 ALS 患者和 19 例非炎症性神经疾病（NIND）患者的血清和脑脊液（CSF）中 IL-17 和 IL-23 的水平，并将其作为对照组进行研究。还将 ALS 患者的 IL-17 和 IL-23 血清和 CSF 水平与疾病持续时间、残疾程度和疾病发病的临床亚型相关联。

结果

与 NIND 患者相比，ALS 患者的血清 IL-17 和 IL-23 水平升高（P = 0.015 和 P = 0.002）。ALS 患者的 CSF 中 IL-17 和 IL-23 水平也升高（P = 0.0006 和 P = 0.000001）。IL-17 和 IL-23 水平与 ALS 患者的疾病持续时间、残疾量表或疾病发病的临床亚型无关。