Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Theriogenology. 2010 Jul 1;74(1):31-9. doi: 10.1016/j.theriogenology.2009.12.016. Epub 2010 Mar 12.
The collection of semen from tomcats by urethral catheterization (CT) after medetomidine administration offers a novel and easy approach to obtain good quality sperm for in vitro fertilization. This study was designed to compare the sperm quality parameters and in vitro fertilizing capacity of CT spermatozoa with those of spermatozoa retrieved after epididymal slicing (EP). Semen was collected in seventeen adult cats by urethral catheterization, after which the cat was orchiectomized. Motility, morphology, plasma membrane integrity, acrosomal status, and in vitro fertilizing capacity of both fresh CT and EP samples were evaluated. The results showed that both total and progressive motility, as well as the percentage of normal spermatozoa, were higher for EP sperm than for CT sperm (P<0.01). Epididymal sperm had a lower percentage of spermatozoa with an intact acrosome (P<0.01), while CT sperm contained more spermatozoa with tail abnormalities (P<0.01). Other morphological parameters, as well as plasma membrane integrity, did not differ (P>0.05) between CT and EP sperm. Nevertheless, no difference (P>0.05) in in vitro fertilizing capacity between spermatozoa collected by means of the two different methods was found. In conclusion, semen collection by means of urethral catheterization after medetomidine administration yields fertilization results similar to epididymal slicing, despite the fact that several sperm variables were different. Since this novel catheterization technique is repeatable, is easy to perform and facilitates semen preparation protocols, it may be preferable for routine IVF experiments with fresh spermatozoa.
通过尿道插管（CT）在给予美托咪定后从公猫中收集精液为获得用于体外受精的高质量精子提供了一种新颖且简单的方法。本研究旨在比较 CT 精子和附睾切片（EP）获得的精子的精子质量参数和体外受精能力。通过尿道插管在 17 只成年猫中收集精液，然后对猫进行去势。评估了新鲜 CT 和 EP 样本的运动性、形态、质膜完整性、顶体状态和体外受精能力。结果表明，EP 精子的总运动力、前向运动力和正常精子的百分比均高于 CT 精子（P<0.01）。附睾精子的顶体完整的精子比例较低（P<0.01），而 CT 精子的尾部异常的精子比例较高（P<0.01）。CT 和 EP 精子的其他形态参数以及质膜完整性无差异（P>0.05）。然而，两种不同方法收集的精子的体外受精能力无差异（P>0.05）。总之，尽管一些精子变量不同，但通过尿道插管在给予美托咪定后收集精液可产生与附睾切片相似的受精结果。由于这种新的插管技术可重复、易于操作且有助于精液制备方案，因此它可能更适合用于新鲜精子的常规 IVF 实验。
