Cunto Marco, Ballotta Giulia, Contri Alberto, Gloria Alessia, Zambelli Daniele
Department of Veterinary Medical Sciences, Alma Mater Studiorum-University of Bologna, Ozzano dell'Emilia, 40064 Bologna, Italy.
Department of Veterinary Medicine, University of Teramo, Loc. Piano d'Accio, 64100 Teramo, Italy.
Animals (Basel). 2025 Feb 11;15(4):504. doi: 10.3390/ani15040504.
The aim of this study was to evaluate the effectiveness of different anesthetic protocols for semen collection from healthy tomcats using Urethral Catheterization after Pharmacological Induction (UrCaPI). Twenty sexually mature tomcats (Felis catus) were included in the study, in which high and low dosages of medetomidine or dexmedetomidine were administrated to collect semen. Ten healthy mature tomcats (Group A) received high dosages of the drugs, and, in particular, five of them were i.m. injected with 120 μg/kg of medetomidine (HMED) on day 1 and with 60 μg/kg of dexmedetomidine (HDEX) after 24 h (day 2). The remaining five tomcats of this group were i.m. injected with 60 μg/kg of dexmedetomidine on day 1 and with 120 μg/kg of medetomidine on day 2. The other ten healthy mature tomcats (Group B) received a low dosage of the same drugs; the first five tomcats were i.m. injected with 50 μg/kg of medetomidine (LMED) on day 1 and with 25 μg/kg of dexmedetomidine (LDEX) on day 2; the others were i.m. injected with 25 μg/kg of dexmedetomidine on day 1 and with 50 μg/kg of medetomidine after 24 h. In both groups, semen collection was performed as soon as the pharmacological effect of the drug was reached. All protocols permitted sperm collection, even if with different results in quality for volume, concentration, total number of spermatozoa, and movement score. Results suggest that both a high dosage of medetomidine and a high dosage of dexmedetomidine could be used for the collection of good-quality semen. Semen volume μL: HMED = 32 μL (25.75-37.5), HDEX = 23 μL (15.25-28). Concentration HMED = 670 × 10/mL (576-990.5), HDEX = 670.5 × 10/mL (536-790). Total number of spermatozoa = 23.24 × 10 (18.37-32.05). Total number of spermatozoa = 13.121 × 10 (10.116-16.83). However, the protocol with dexmedetomidine could not always guarantee an adequate sedation for urethral catheterization, so using a high dosage of medetomidine is still the better way to collect high quality semen with the UrCaPI technique. The outcome of the LMED and LDEX protocols was generally unsatisfactory regarding both level of sedation and semen collection in terms of volume (LMED = 3 μL (4-7.5); LDEX = 6 μL (4-7)), concentration (LMED = 215 × 10/mL (157-248); LDEX = 27.05 × 10/mL (0.040-110), total number of spermatozoa (LMED = 0.88 × 10 (0.581-1.38); LDEX = 0.16 × 10 (0.0001-0.80), and movement score (LMED = 3 (2-3); LDEX = 2 (0-2.75)).
本研究的目的是评估不同麻醉方案对采用药理诱导后尿道插管法（UrCaPI）从健康雄猫采集精液的有效性。20只性成熟的雄猫（家猫）被纳入该研究，研究中给予高剂量和低剂量的美托咪定或右美托咪定以采集精液。10只健康成熟的雄猫（A组）接受高剂量的药物，具体而言，其中5只在第1天肌肉注射120μg/kg的美托咪定（HMED），并在24小时后（第2天）注射60μg/kg的右美托咪定（HDEX）。该组其余5只雄猫在第1天肌肉注射60μg/kg的右美托咪定，在第2天注射120μg/kg的美托咪定。另外10只健康成熟的雄猫（B组）接受低剂量的相同药物；前5只雄猫在第1天肌肉注射50μg/kg的美托咪定（LMED），在第2天注射25μg/kg的右美托咪定（LDEX）；其余的在第1天肌肉注射25μg/kg的右美托咪定，并在24小时后注射50μg/kg的美托咪定。在两组中，一旦达到药物的药理作用，就进行精液采集。所有方案均允许采集精子，尽管在精液量、浓度、精子总数和活力评分的质量方面结果不同。结果表明，高剂量的美托咪定和高剂量的右美托咪定均可用于采集高质量的精液。精液量（μL）：HMED = 32μL（25.75 - 37.5），HDEX = 23μL（15.25 - 28）。浓度：HMED = 670×10⁶/mL（576 - 990.5），HDEX = 670.5×10⁶/mL（536 - 790）。精子总数 = 23.24×10⁶（18.37 - 32.05）。精子总数 = 13.121×10⁶（10.116 - 16.83）。然而，右美托咪定方案不能始终保证足够的镇静以进行尿道插管，因此使用高剂量的美托咪定仍然是采用UrCaPI技术采集高质量精液的更好方法。低剂量美托咪定（LMED）和低剂量右美托咪定（LDEX）方案在镇静水平和精液采集方面的结果总体上不令人满意，在精液量方面（LMED = 3μL（4 - 7.5）；LDEX = 6μL（4 - 7）），浓度方面（LMED = 215×10⁶/mL（157 - 248）；LDEX = 27.05×10⁶/mL（0.040 - 110）），精子总数方面（LMED = 0.88×10⁶（0.581 - 1.38）；LDEX = 0.16×10⁶（0.0001 - 0.80）），以及活力评分方面（LMED = 3（2 - 3）；LDEX = 2（0 - 2.75））。
