Zhou Qian Yun, Liu Ding, Huang Shi Feng, Wen Yang An, Luo Peng, Xiang Yu, Sun Shan, Dong Yu Fang, Zhang Li Ping
Department of Clinical Laboratory, The First Affiliated Hospital, Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing, 400016, PR China.
Exp Toxicol Pathol. 2011 May;63(4):307-10. doi: 10.1016/j.etp.2010.02.003. Epub 2010 Mar 9.
C/EBPα transcription factor is a key regulator in liver biology and was preliminarily shown to be down-regulated in hypoxic primary rat hepatocytes. The aim of this study was to explore the possible association between C/EBPα expression level and hepatocyte viability in both the in-vitro cultured hypoxic rat primary hepatocytes and two models of acute liver hypoxia induced by carbon tetrachloride or Fas antibody. C/EBPα mRNA was significantly down-regulated under hypoxic conditions both in vitro and in vivo, which was paralleled by a similar decrease in hepatocyte viability and partially reversed by 3D matrix and dexamethasone. These results suggested that C/EBPα down-regulation may be one mechanism of reduced hepatocyte viability in these settings.
C/EBPα转录因子是肝脏生物学中的关键调节因子，初步研究表明其在缺氧的原代大鼠肝细胞中表达下调。本研究的目的是探讨在体外培养的缺氧大鼠原代肝细胞以及四氯化碳或Fas抗体诱导的两种急性肝缺氧模型中，C/EBPα表达水平与肝细胞活力之间可能存在的关联。在体外和体内缺氧条件下，C/EBPα mRNA均显著下调，同时肝细胞活力也出现类似下降，而三维基质和地塞米松可部分逆转这种下降。这些结果表明，C/EBPα下调可能是这些情况下肝细胞活力降低的一种机制。
