Osborne M P, Wong G Y, Asina S, Old L J, Cote R J, Rosen P P
Breast Cancer Research Laboratory, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Cancer Res. 1991 May 15;51(10):2706-9.
We have previously shown that occult micrometastases can be detected in the bone marrow of breast cancer patients, at the time of initial treatment, using a panel of epithelial specific monoclonal antibodies indirectly labeled with fluorescein. These monoclonal antibodies permit us to detect cancer cells at at concentration of two/million normal bone marrow cells. Immunofluorescence carries the disadvantage that detailed morphological examination of detected cells cannot be accomplished. A modification of the alkaline phosphatase anti-alkaline phosphatase method has been used to detect cancer cells and to observe their morphology in human bone marrow. The sensitivity of this method has been examined using an established human metastatic breast cancer cell line (MCF-7) mixed with normal bone marrow cells at various dilutions from 400 cancer cells/10(6) marrow cells to 10 cancer cells/10(6) marrow cells. The number of immunocytochemically stained MCF-7 cells counted at each concentration was related to the concentration by a simple nonlinear statistical model. At a concentration of 10 cancer cells/10(6) bone marrow cells, the model shows that this method has the sensitivity to detect between four and six MCF-7 cells 95% of the time. Extrapolation, using this model, predicts that at the very low concentration of one cancer cell/10(6) marrow cells, there is a 95% chance of detecting the cancer cell. This assay may be a very sensitive method for detecting cancer cells in the bone marrow of breast cancer patients.
我们之前已经表明，在乳腺癌患者初始治疗时，使用一组间接标记有荧光素的上皮特异性单克隆抗体，可以在其骨髓中检测到隐匿性微转移。这些单克隆抗体使我们能够在每两百万个正常骨髓细胞的浓度下检测到癌细胞。免疫荧光的缺点是无法对检测到的细胞进行详细的形态学检查。一种改良的碱性磷酸酶抗碱性磷酸酶方法已被用于检测人骨髓中的癌细胞并观察其形态。使用已建立的人转移性乳腺癌细胞系（MCF - 7）与正常骨髓细胞以从400个癌细胞/10⁶个骨髓细胞到10个癌细胞/10⁶个骨髓细胞的各种稀释度混合，来检测该方法的灵敏度。在每个浓度下计数的免疫细胞化学染色的MCF - 7细胞数量通过一个简单的非线性统计模型与浓度相关。在10个癌细胞/10⁶个骨髓细胞的浓度下，该模型表明该方法在95%的时间内有检测到4至6个MCF - 7细胞的灵敏度。使用该模型进行外推预测，在极低的浓度1个癌细胞/10⁶个骨髓细胞下，有95%的机会检测到癌细胞。该检测方法可能是一种检测乳腺癌患者骨髓中癌细胞的非常灵敏的方法。
