[黑胸散白蚁肠道共生古菌的系统发育分析]
[Phylogenetic analysis of symbiotic archaea in the gut of Reticulitermes chinensis Snyder].
作者信息
Shi Yu, Zhang Yanhong, Yang Hong
机构信息
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China.
出版信息
Wei Sheng Wu Xue Bao. 2009 Dec;49(12):1655-9.
OBJECTIVE
To analyze the phylotypes of symbiotic archaea in the gut of Reticulitermes chinensis Snyder by using non-cultivating method.
METHODS
We amplified 16S rRNA genes of the symbiotic archaea from the DNA extract of the whole gut with the universal archaeal 16S rDNA primer. A clone library of archaeal 16S rDNA was established and selected clones were sequenced and phylogeneticly analyzed.
RESULTS
Five 16S rDNA sequences with the similarity between 93.2% and 99.2% to each other were obtained from Reticulitermes chinensis Snyder. Phylogenetic analysis showed that the five clones were clustered with Methanobrevibacter clones or isolates obtained from Reticulitermes speratus and Reticulitermes flavipes.
CONCLUSION
The results showed that symbiotic archaea in the gut of Reticulitermes chinensis were affiliated with Methanobrevibacter.
目的
采用非培养方法分析黑胸散白蚁肠道中共生古菌的系统发育型。
方法
用通用古菌16S rDNA引物从整个肠道的DNA提取物中扩增共生古菌的16S rRNA基因。建立古菌16S rDNA克隆文库，对筛选出的克隆进行测序和系统发育分析。
结果
从黑胸散白蚁中获得了5条16S rDNA序列，它们之间的相似性为93.2%至99.2%。系统发育分析表明，这5个克隆与从日本散白蚁和黄胸散白蚁中获得的甲烷短杆菌克隆或分离株聚类在一起。
结论
结果表明，黑胸散白蚁肠道中的共生古菌属于甲烷短杆菌属。
Zotero 插件