Meng Xiangwei, Mao Zhenchuan, Chen Guohua, Yang Yuhong, Xie Bingyan
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Wei Sheng Wu Xue Bao. 2009 Aug;49(8):994-1002.
In order to study the diversity of archaea and ammonia-oxidizing archaea (AOA) of the alp prairie soil in Mila Mountain of Tibet.
Total microbial DNA was directly extracted from the alp prairie of Mila Mountain. The clone library of 16S rRNA genes and amoA genes were amplified by PCR with universal primer sets. The sequences of archaea and AOA were defined into operational taxonomic units (OTUs) according to the 97% similarity threshold for OTU assignment was performed using the software program DOTUR.
Phylogenetic analysis revealed archaea in the soil of Mila Mountain including the Crenarchaeota (71.7%) and unclassified-Archaea (28.3%) phyla. All the Crenarchaeota belong to the Thermoprotei. Phylogenetic analysis revealed AOA in the alp prairie soil of Mila Mountain belonged to the kingdom Crenarchaeota. Archaea and AOA species composition from Mila Mountain included 64 OTUs and 75 OTUs.
These findings show prolific archaeal diversity in the alp prairie soil of Mila Mountain, where they may be actively involved in nitrification.
研究西藏米拉山高寒草原土壤中古菌和氨氧化古菌（AOA）的多样性。
直接从米拉山高寒草原土壤中提取总微生物DNA。使用通用引物对通过PCR扩增16S rRNA基因和amoA基因的克隆文库。根据97%的相似性阈值，将古菌和AOA的序列定义为操作分类单元（OTU），使用软件程序DOTUR进行OTU划分。
系统发育分析表明，米拉山土壤中的古菌包括泉古菌门（71.7%）和未分类古菌（28.3%）。所有泉古菌都属于热变形菌纲。系统发育分析表明，米拉山高寒草原土壤中的AOA属于泉古菌门。米拉山的古菌和AOA物种组成包括64个OTU和75个OTU。
这些发现表明米拉山高寒草原土壤中古菌多样性丰富，它们可能积极参与硝化作用。
