Gu Xiaohua, Duan Xujie, Tan Wensong, Zhang Xu
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Sheng Wu Gong Cheng Xue Bao. 2009 Nov;25(11):1671-8.
Agitation plays an important role in the hyaluronic acid (HA) fermentation process. However, views about the effect of agitation on HA production remain controversial. We investigated the effect of agitation on cell growth and HA synthesis during HA fermentation process by using Computational Fluid Dynamics (CFD) technology. The results showed that the biomass and HA yield changed a little with the increase of impeller speed, but the HA molecular weight firstly increased and then decreased. The results of phase agitation control strategy demonstrated that the influence of agitation on the HA molecular weight mainly exhibited at the stage of HA synthesis. Moreover, the CFD simulation results indicated that when impeller speed increased, the mixing time reduced while the shear rate increased significantly. The removal of anchor could moderate the contradiction between the mixing time and shear rate, and finally the HA molecular weight increased by 23.9%. The results of this work could provide guidelines for optimizing the HA fermentation, as well as the bioreactor design and scaling up.
搅拌在透明质酸（HA）发酵过程中起着重要作用。然而，关于搅拌对HA产量影响的观点仍存在争议。我们通过使用计算流体动力学（CFD）技术研究了搅拌在HA发酵过程中对细胞生长和HA合成的影响。结果表明，随着搅拌桨转速的增加，生物量和HA产量变化不大，但HA分子量先增加后降低。阶段性搅拌控制策略的结果表明，搅拌对HA分子量的影响主要表现在HA合成阶段。此外，CFD模拟结果表明，当搅拌桨转速增加时，混合时间缩短，而剪切速率显著增加。去除锚式搅拌桨可以缓解混合时间和剪切速率之间的矛盾，最终HA分子量提高了23.9%。这项工作的结果可为优化HA发酵、生物反应器设计及放大提供指导。
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