[培养条件对L(+)-乳酸光学纯度的影响]

[Effects of cultivation conditions on the optical purity of L(+)-lactic acid].

作者信息

Meng Wu, Li Shizhong, Feng Wentao, Zhang Hanxing, Wang Ruiming

机构信息

College of Food and Bioengineering, Shandong Institute of Light Industry, Jinan 250353, China

出版信息

Sheng Wu Gong Cheng Xue Bao. 2009 Nov;25(11):1679-83.

PMID:20222467

Abstract

The effect of cultivation conditions on the optical purity of L(+)-lactic acid produced by Rhizopus oryzae HZS6 was investigated. The isomeric composition of lactic acid was influenced by the supplementation of L(+)-lactic acid to fermentation medium. L(+)-isomer increased with the dosage, no D(-)-lactic acid was observed when the concentration of supplemented L(+)-lactic acid in matrix was > or = 1.5 g/L. However, the L(+)-lactic acid yield, biomass and glucose conversion rate decreased with the dosage. With the same method, the supplementation of L(+)-lactic to substrate had no influence on isomeric composition of lactic acid by Lactobacillus and Escherichia coli.

摘要

研究了培养条件对米根霉HZS6产L(+)-乳酸光学纯度的影响。发酵培养基中添加L(+)-乳酸会影响乳酸的异构体组成。L(+)-异构体随添加量增加，当基质中添加的L(+)-乳酸浓度≥1.5 g/L时，未观察到D(-)-乳酸。然而，L(+)-乳酸产量、生物量和葡萄糖转化率随添加量降低。采用相同方法，向底物中添加L(+)-乳酸对乳酸杆菌和大肠杆菌产生的乳酸异构体组成没有影响。