使用CHROMagar SA培养基对海水中的金黄色葡萄球菌进行定量分析。
Quantitation of Staphylococcus aureus in seawater using CHROMagar SA.
作者信息
Tice Alan D, Pombo David, Hui Jennifer, Kurano Michelle, Bankowski Matthew J, Seifried Steven E
机构信息
Department of Medicine, John A. Burns School of Medicine, University of Hawai'i at Manoa, Honolulu, HI, USA.
出版信息
Hawaii Med J. 2010 Jan;69(1):8-12.
Abstract
A microbiological algorithm has been developed to analyze beach water samples for the determination of viable colony forming units (CFU) of Staphylococcus aureus (S. aureus). Membrane filtration enumeration of S. aureus from recreational beach waters using the chromogenic media CHROMagar SA alone yields a positive predictive value (PPV) of 70%. Presumptive CHROMagar SA colonies were confirmed as S. aureus by 24-hour tube coagulase test. Combined, these two tests yield a PPV of 100%. This algorithm enables accurate quantitation of S. aureus in seawater in 72 hours and could support risk-prediction processes for recreational waters. A more rapid protocol, utilizing a 4-hour tube coagulase confirmatory test, enables a 48-hour turnaround time with a modest false negative rate of less than 10%.
摘要
已开发出一种微生物学算法，用于分析海滩水样，以测定金黄色葡萄球菌（S. aureus）的活菌菌落形成单位（CFU）。仅使用显色培养基CHROMagar SA对休闲海滩水域中的金黄色葡萄球菌进行膜过滤计数，其阳性预测值（PPV）为70%。通过24小时试管凝固酶试验将CHROMagar SA上的疑似菌落确认为金黄色葡萄球菌。这两种测试结合使用时，PPV为100%。该算法能够在72小时内准确测定海水中金黄色葡萄球菌的数量，并可为休闲水域的风险预测过程提供支持。一种更快速的方案，采用4小时试管凝固酶确证试验，可实现48小时的周转时间，假阴性率适中，低于10%。
相似文献
1
Quantitation of Staphylococcus aureus in seawater using CHROMagar SA.
Hawaii Med J. 2010 Jan;69(1):8-12.
2
Performance of CHROMagar Staph aureus and CHROMagar MRSA for detection of Staphylococcus aureus in seawater and beach sand--comparison of culture, agglutination, and molecular analyses.
Water Res. 2009 Nov;43(19):4802-11. doi: 10.1016/j.watres.2009.06.025. Epub 2009 Jun 18.
3
Performance of the chromogenic medium CHROMagar Staph Aureus and the Staphychrom coagulase test in the detection and identification of Staphylococcus aureus in clinical specimens.
J Clin Microbiol. 2001 Jul;39(7):2581-3. doi: 10.1128/JCM.39.7.2581-2583.2001.
4
[Laboratory-based evaluation of a selective X-SA agar medium supplemented with chromogenic substrate for Staphylococcus aureus].
Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi. 2005;16(2):135-45.
5
The application of peptide nucleic acid probes for rapid detection and enumeration of eubacteria, Staphylococcus aureus and Pseudomonas aeruginosa in recreational beaches of S. Florida.
J Microbiol Methods. 2004 May;57(2):157-62. doi: 10.1016/j.mimet.2003.12.005.
6
[Identification of Staphylococcus aureus within 2 hours in blood culture broths].
Ann Biol Clin (Paris). 1999 Mar-Apr;57(2):197-200.
7
Comparison of the BBL CHROMagar Staph aureus agar medium to conventional media for detection of Staphylococcus aureus in respiratory samples.
J Clin Microbiol. 2004 Aug;42(8):3566-9. doi: 10.1128/JCM.42.8.3566-3569.2004.
8
Optimal detection of Staphylococcus aureus from clinical specimens using a new chromogenic medium.
Diagn Microbiol Infect Dis. 2004 Aug;49(4):243-7. doi: 10.1016/j.diagmicrobio.2004.02.009.
9
Use of peptide nucleic acid probes for rapid detection and enumeration of viable bacteria in recreational waters and beach sand.
Methods Mol Biol. 2006;345:131-40. doi: 10.1385/1-59745-143-6:131.
10
A multi-beach study of Staphylococcus aureus, MRSA, and enterococci in seawater and beach sand.
Water Res. 2012 Sep 1;46(13):4195-207. doi: 10.1016/j.watres.2012.04.001. Epub 2012 Apr 21.
引用本文的文献
1
The presence of antibiotics and multidrug-resistant Staphylococcus aureus reservoir in a low-order stream spring in central Brazil.
Braz J Microbiol. 2023 Jun;54(2):997-1007. doi: 10.1007/s42770-023-00973-9. Epub 2023 Apr 22.
2
Prevalence and Characterization of and Methicillin-Resistant on Public Recreational Beaches in Northeast Ohio.
Geohealth. 2017 Dec 14;1(10):320-332. doi: 10.1002/2017GH000106. eCollection 2017 Dec.
3
Comparison of Multi-Drug Resistant Environmental Methicillin-Resistant Staphylococcus aureus Isolated from Recreational Beaches and High Touch Surfaces in Built Environments.
Front Microbiol. 2013 Apr 4;4:74. doi: 10.3389/fmicb.2013.00074. eCollection 2013.
4
Human-associated methicillin-resistant Staphylococcus aureus from a subtropical recreational marine beach.
Microb Ecol. 2013 May;65(4):1039-51. doi: 10.1007/s00248-013-0216-1. Epub 2013 Apr 4.
5
Spatial and temporal variation in indicator microbe sampling is influential in beach management decisions.
Water Res. 2012 May 1;46(7):2237-46. doi: 10.1016/j.watres.2012.01.040. Epub 2012 Feb 4.
6
Methicillin-resistant Staphylococcus aureus from Northwest marine and freshwater recreational beaches.
FEMS Microbiol Ecol. 2012 Feb;79(2):412-20. doi: 10.1111/j.1574-6941.2011.01229.x. Epub 2011 Nov 15.
7
Quantification of methicillin-resistant Staphylococcus aureus strains in marine and freshwater samples by the most-probable-number method.
Appl Environ Microbiol. 2011 May;77(10):3541-3. doi: 10.1128/AEM.00015-11. Epub 2011 Mar 25.
8
Shedding of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus from adult and pediatric bathers in marine waters.
BMC Microbiol. 2011 Jan 6;11(1):5. doi: 10.1186/1471-2180-11-5.
本文引用的文献
1
A review of community-associated methicillin-resistant Staphylococcus aureus skin and soft tissue infections.
Pediatr Infect Dis J. 2008 Jan;27(1):1-7. doi: 10.1097/INF.0b013e31815819bb.
2
Invasive methicillin-resistant Staphylococcus aureus infections in the United States.
JAMA. 2007 Oct 17;298(15):1763-71. doi: 10.1001/jama.298.15.1763.
3
Microbial structuring of marine ecosystems.
Nat Rev Microbiol. 2007 Oct;5(10):782-91. doi: 10.1038/nrmicro1747.
4
Quantitative evaluation of bacteria released by bathers in a marine water.
Water Res. 2007 Jan;41(1):3-10. doi: 10.1016/j.watres.2006.10.005. Epub 2006 Nov 17.
5
Rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time PCR.
Appl Environ Microbiol. 2007 Jan;73(1):92-100. doi: 10.1128/AEM.01772-06. Epub 2006 Oct 20.
6
Isolates of Staphylococcus aureus and saprophyticus resistant to antimicrobials isolated from the Lebanese aquatic environment.
Mar Pollut Bull. 2006 Aug;52(8):912-9. doi: 10.1016/j.marpolbul.2005.12.008. Epub 2006 Feb 20.
7
Evaluation of six agglutination tests for Staphylococcus aureus identification depending upon local prevalence of meticillin-resistant S. aureus (MRSA).
J Med Microbiol. 2006 Mar;55(Pt 3):283-290. doi: 10.1099/jmm.0.46225-0.
8
Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA).
J Antimicrob Chemother. 2005 Dec;56(6):1000-18. doi: 10.1093/jac/dki372. Epub 2005 Nov 17.
9
Molecular diversity and ecology of microbial plankton.
Nature. 2005 Sep 15;437(7057):343-8. doi: 10.1038/nature04158.
10
Genomic perspectives in microbial oceanography.
Nature. 2005 Sep 15;437(7057):336-42. doi: 10.1038/nature04157.
Zotero 插件