Gak E, Yaniv A, Sherman L, Ianconescu M, Tronick S R, Gazit A
Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Israel.
Gene. 1991 Mar 15;99(2):157-62. doi: 10.1016/0378-1119(91)90122-r.
The lymphoproliferative disease virus (LPDV) is the etiological agent of a lymphoproliferative disease that naturally occurs in turkeys. Recently, we have cloned the LPDV provirus and established it as a replication-competent genome devoid of a viral oncogene [Gak et al., J. Virol. 63 (1989) 2877-2880]. This report presents the nucleotide sequence of its long terminal repeat (LTR) and establishes it as a potent transcriptional element. Several features of the LPDV LTR were similar to those found in the LTRs of the avian sarcoma-leukemia viruses (ASLV) and include the primer-binding site (tRNATrp), the polypurine tract, the organization of the polyadenylation signal, the complexities of the U3, R and U5 regions, as well as a potential secondary structure in U5-R. The LTR sequence diverges significantly from the ASLV LTRs, which share a common structure and have extensive sequence homology mainly in the R and U5 domains. These findings support the conclusion that LPDV represents a distinct class of avian retrovirus, evolutionarily related to the ASLV family.
淋巴细胞增生性疾病病毒（LPDV）是一种自然发生于火鸡的淋巴细胞增生性疾病的病原体。最近，我们克隆了LPDV前病毒，并将其确立为一个具有复制能力且不含病毒癌基因的基因组[Gak等人，《病毒学杂志》63(1989)2877 - 2880]。本报告展示了其长末端重复序列（LTR）的核苷酸序列，并将其确立为一种有效的转录元件。LPDV LTR的几个特征与禽肉瘤 - 白血病病毒（ASLV）的LTR中发现的特征相似，包括引物结合位点（tRNATrp）、多聚嘌呤序列、多聚腺苷酸化信号的组织方式、U3、R和U5区域的复杂性，以及U5 - R中的潜在二级结构。LTR序列与ASLV的LTR有显著差异，ASLV的LTR具有共同结构，且主要在R和U5结构域有广泛的序列同源性。这些发现支持了LPDV代表一类与ASLV家族在进化上相关的独特禽逆转录病毒的结论。
