Du Zhong-Wei, Zhang Su-Chun
Departments of Anatomy and Neurology, School of Medicine and Public Health, WiCell Institute, University of Wisconsin, Madison, WI, USA.
Methods Mol Biol. 2010;614:127-34. doi: 10.1007/978-1-60761-533-0_8.
Human Embryonic stem cells (hESCs) offer an invaluable tool for revealing human biology and a potential source of functional cells/tissues for regenerative medicine. The utility of hESCs will likely be significantly enhanced and broadened by our ability to build versatile genetically modified hESC lines. Here, we describe an efficient lentiviral vector mediated method to establish stable transgenic hESCs.
人类胚胎干细胞（hESCs）为揭示人类生物学提供了一种宝贵的工具，也是再生医学中功能性细胞/组织的潜在来源。我们构建通用基因修饰hESC系的能力可能会显著增强和拓宽hESCs的用途。在此，我们描述了一种有效的慢病毒载体介导的方法来建立稳定的转基因hESCs。
