The Key Sericultural Laboratory of Agricultural Ministry, College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China.
BMC Genomics. 2010 Mar 15;11:173. doi: 10.1186/1471-2164-11-173.
Insect cuticle plays essential roles in many physiological functions. During molting and metamorphosis tremendous changes occur in silkworm cuticle where multiple proteins exist and genes encoding them constitute about 1.5% of all Bombyx mori genes.
In an effort to determine their expression profiles, a microarray-based investigation was carried out using mRNA collected from larvae to pupae. The results showed that a total of 6676 genes involved in various functions and physiological pathways were activated. The vast majority (93%) of cuticular protein genes were expressed in selected stages with varying expression patterns. There was no correlation between expression patterns and the presence of conserved motifs. Twenty-six RR genes distributed in chromosome 22 were co-expressed at the larval and wandering stages. The 2 kb upstream regions of these genes were further analyzed and three putative elements were identified.
Data from the present study provide, for the first time, a comprehensive expression profile of genes in silkworm epidermal tissues and evidence that putative elements exist to allow massive production of mRNAs from specific cuticular protein genes.
昆虫的外骨骼在许多生理功能中起着至关重要的作用。在蜕皮和变态过程中，家蚕外骨骼发生了巨大的变化，存在多种蛋白质，编码这些蛋白质的基因约占家蚕所有基因的 1.5%。
为了确定它们的表达谱，我们使用从小蚕到蛹收集的 mRNA 进行了基于微阵列的研究。结果表明，共有 6676 个参与各种功能和生理途径的基因被激活。绝大多数（93%）的表皮蛋白基因在选定的阶段以不同的表达模式表达。表达模式与保守基序的存在之间没有相关性。26 个 RR 基因分布在 22 号染色体上，在幼虫和游走阶段共同表达。进一步分析了这些基因的 2kb 上游区域，鉴定出了三个假定的元件。
本研究的数据首次提供了家蚕表皮组织中基因的全面表达谱，并为特定表皮蛋白基因大量产生 mRNA 提供了证据，表明存在假定的元件。
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