State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
Insect Sci. 2020 Oct;27(5):998-1018. doi: 10.1111/1744-7917.12711. Epub 2019 Aug 6.
Recently, parasitoid wasp species Microplitis mediator has evoked increasing research attention due to its possible use in the control of Lepidoptera insects. Because insect development involves changes in cuticle composition, identification and expression analysis of M. mediator cuticular proteins may clarify the mechanisms involved in parasite development processes. We found 70 cuticular proteins from the M. mediator transcriptome and divided them into seven distinct families. Expression profiling indicated that most of these cuticular protein genes have expression peaks specific for one particular developmental stage of M. mediator. Eggs and pupae have the highest number of transcriptionally active cuticular protein genes (47 and 52 respectively). Only 12 of these genes maintained high expression activity during late larval development. Functional analysis of two larval proteins, MmCPR3 and MmCPR14, suggested their important role in the proper organization of the cuticle layers of larvae. During M. mediator larval development, normal cuticle formation can be supported by a limited number of cuticular proteins.
最近，由于其在鳞翅目昆虫控制中的潜在应用，寄生蜂物种 Mediator 小蜂引起了越来越多的研究关注。由于昆虫的发育涉及表皮组成的变化，因此鉴定和表达分析 Mediator 小蜂的表皮蛋白可能阐明寄生虫发育过程中涉及的机制。我们从 Mediator 的转录组中发现了 70 种表皮蛋白，并将它们分为七个不同的家族。表达谱分析表明，这些表皮蛋白基因中的大多数都有特定于 Mediator 特定发育阶段的表达峰。卵和蛹具有转录活性最高的表皮蛋白基因（分别为 47 和 52 个）。在幼虫晚期发育过程中，只有 12 个基因保持高表达活性。对两个幼虫蛋白 MmCPR3 和 MmCPR14 的功能分析表明，它们在幼虫表皮层的正常组织中起着重要作用。在 Mediator 小蜂幼虫发育过程中，有限数量的表皮蛋白就可以支持正常的表皮形成。
