Jin Jin, Zhang Yang, Hu Wen-xiang, Zhang Zheng-yao, Xu Nan-nan, Zhou Qiu-li
Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Changchun 130022, Jilin Province, China.
Zhong Xi Yi Jie He Xue Bao. 2010 Mar;8(3):275-9. doi: 10.3736/jcim20100313.
To observe the effects of Huanglian Jiedu Decoction (HJD), a compound traditional Chinese herbal medicine, on lipid metabolism and its related gene expressions in rats with hyperlipidemia.
Fifty SD rats were randomly divided into normal control group, untreated group, Lipitor (atorvastatin) group, and low- and high-dose HJD groups. Except the normal control group, rats in the other groups were fed with high-fat diet to induce hyperlipidemia. Then the rats were administered with corresponding drugs for 8 weeks. After treatment, the serum levels of total cholesterol (TC), triacylglycerol (TAG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were assayed. The activities of lipoprotein lipase (LPL) and hepatic lipase (HL) in liver tissues were measured. Low-density lipoprotein receptor (LDLR) and peroxisome proliferator-activated receptor gamma (PPARgamma) mRNA expressions in liver tissues were determined by reverse transcription-polymerase chain reaction.
Compared with the normal control group, the levels of serum TC, TAG and LDL-C in the untreated group were increased and the level of serum HDL-C was reduced. The activities of LPL and HL and expressions of LDLR and PPARgamma mRNAs in the untreated group were lower than those in the normal control group. After treatment, high-dose HJD significantly improved hyperlipemia by decreasing TC, TAG and LDL-C and increasing HDL-C. The activities of LPL and HL and expression levels of LDLR and PPARgamma mRNAs in liver tissues were also markedly enhanced in the high-dose HJD group as compared with those in the untreated group.
HJD can activate the activity of lipid metabolism enzyme, and enhance the expressions of LDLR and PPARgamma mRNAs to modulate the lipid metabolic disorders in rats with hyperlipidemia.
观察中药复方黄连解毒汤对高脂血症大鼠脂质代谢及其相关基因表达的影响。
将50只SD大鼠随机分为正常对照组、未治疗组、立普妥（阿托伐他汀）组、黄连解毒汤低剂量组和高剂量组。除正常对照组外，其他组大鼠给予高脂饮食诱导高脂血症。然后给予相应药物治疗8周。治疗后，检测血清总胆固醇（TC）、三酰甘油（TAG）、低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）水平。测定肝组织中脂蛋白脂肪酶（LPL）和肝脂肪酶（HL）的活性。采用逆转录-聚合酶链反应法检测肝组织中低密度脂蛋白受体（LDLR）和过氧化物酶体增殖物激活受体γ（PPARγ）mRNA的表达。
与正常对照组相比，未治疗组大鼠血清TC、TAG和LDL-C水平升高，血清HDL-C水平降低。未治疗组LPL和HL的活性以及LDLR和PPARγ mRNA的表达均低于正常对照组。治疗后，黄连解毒汤高剂量组通过降低TC、TAG和LDL-C以及升高HDL-C显著改善了高脂血症。与未治疗组相比，黄连解毒汤高剂量组肝组织中LPL和HL的活性以及LDLR和PPARγ mRNA的表达水平也明显增强。
黄连解毒汤可激活脂质代谢酶的活性，增强LDLR和PPARγ mRNA的表达，调节高脂血症大鼠的脂质代谢紊乱。
