佛波酯TPA抑制静止的BALB/c 3T3小鼠成纤维细胞中不同促细胞分裂剂对布美他尼敏感的Na+/K+/Cl-转运体的刺激作用。
Phorbol ester TPA inhibits the stimulation of bumetanide-sensitive Na+/K+/Cl- transporter by different mitogens in quiescent BALB/c 3T3 mouse fibroblasts.
作者信息
Snyder D, Markus M, Atlan H, Panet R
机构信息
Department of Medical Biophysics and Nuclear Medicine, Hadassah University Hospital, Jerusalem, Israel.
出版信息
J Cell Physiol. 1991 Mar;146(3):466-72. doi: 10.1002/jcp.1041460318.
In this study we examined the effect of the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on the bumetanide-sensitive Na+/K+/Cl- transporter in quiescent BALB/c 3T3 cells. We have shown that exposure of quiescent BALB/c 3T3 cultures to phorbol ester did not inhibit the basal bumetanide-sensitive Rb+ influx or efflux. In fact, at high concentration (100 ng/ml), TPA slightly stimulated the bumetanide-sensitive Rb+ influx and efflux. However, when the quiescent cultures were stimulated by serum or by defined growth factors, the stimulated fraction of the bumetanide-sensitive Rb+ influx was drastically inhibited by exposure of the cells to the phorbol ester TPA. Based on the above findings, we propose that activation of protein kinase C by the phorbol ester TPA does not inhibit the Na+/K+/Cl- cotransport activity; however it does suppress only the growth-factors-stimulated fraction of the cotransport in quiescent BALB/c 3T3 cells. These data propose that activation of kinase C has a regulatory feedback effect on the stimulation of the Na+/K+/Cl- cotransport activity by growth factors.
在本研究中，我们检测了佛波酯12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）对静止的BALB/c 3T3细胞中布美他尼敏感的Na⁺/K⁺/Cl⁻转运体的影响。我们发现，将静止的BALB/c 3T3培养物暴露于佛波酯并不会抑制基础的布美他尼敏感的Rb⁺内流或外流。事实上，在高浓度（100 ng/ml）时，TPA会轻微刺激布美他尼敏感的Rb⁺内流和外流。然而，当静止培养物受到血清或特定生长因子刺激时，细胞暴露于佛波酯TPA会显著抑制布美他尼敏感的Rb⁺内流的刺激部分。基于上述发现，我们提出佛波酯TPA激活蛋白激酶C并不会抑制Na⁺/K⁺/Cl⁻共转运活性；然而，它仅抑制静止的BALB/c 3T3细胞中生长因子刺激的共转运部分。这些数据表明激酶C的激活对生长因子刺激的Na⁺/K⁺/Cl⁻共转运活性具有调节反馈作用。
Zotero 插件