Université Pierre et Marie Curie, Paris VI, CNRS, Marine Plants and Biomolecules, UMR 7139, Station Biologique, BP 74, F-29680 Roscoff Cedex, France.
Carbohydr Res. 2010 May 7;345(7):934-40. doi: 10.1016/j.carres.2010.02.014. Epub 2010 Feb 19.
Hybrid iota-/nu-carrageenan was water-extracted from Eucheuma denticulatum and incubated with Alteromonas fortis iota-carrageenase. The degradation products were then separated by anion-exchange chromatography. The three most abundant fractions of hybrid iota-/nu-carrageenan oligosaccharides were purified and their structures were analyzed by NMR. The smallest hybrid was an octasaccharide with a iota-iota-nu-iota structure. The second fraction was composed of two decasaccharides with iota-iota-iota-nu-iota and iota-[iota/nu]-iota-iota structures. The third fraction was a mixture of dodecasaccharides which contained at least a iota-iota-iota-iota-nu-iota oligosaccharide. The carbon and proton NMR spectra of the octasaccharides were completely assigned, thereby completely attributing the nu-carrabiose moiety for the first time.
杂合的 ι-/ν-卡拉胶是从角叉菜中用水提取的，并与交替假单胞菌 ι-卡拉胶酶孵育。然后通过阴离子交换色谱分离降解产物。从杂合的 ι-/ν-卡拉胶低聚糖中纯化出三种最丰富的级分，并通过 NMR 分析其结构。最小的杂合是具有 ι-ι-ν-ι 结构的八聚糖。第二部分由两个具有 ι-ι-ι-ν-ι 和 ι-[ι/ν]-ι-ι 结构的十聚糖组成。第三部分是十二聚糖的混合物，其中至少含有一个 ι-ι-ι-ι-ν-ι 低聚糖。八聚糖的碳和质子 NMR 谱完全归属，从而首次完全归属了 ν-卡拉双糖部分。
