Department of Medicinal Biochemistry, Faculty of Pharmaceutial Sciences, Okayama University, Tsushima-naka 1-1, Okayama 700, Japan.
Biochem Pharmacol. 1980 Mar 1;29(5):741-5. doi: 10.1016/0006-2952(80)90550-x.
Incubation of rat peritoneal mast cells with hydrogen peroxide results in a marked release of histamine. Maximal release is observed with 0.05-0.1 mM H(2)O(2), but higher concentrations of H(2)O(2) instead suppresses the release. Histamine release starts after about 2 min of lag time and reaches a plateau in about 10 min. Hydrogen peroxide-induced release does not exceed 50-60 per cent of total histamine if the incubations are prolonged or additional H(2)O(2) is given at 10 min. This would be explained by the fact that H(2)O(2) causes impairment of the histamine releasing system of mast cells simultaneously with the release of histamine. Hydrogen peroxide-induced release is not due to nonspecific lysis of the cells because lactate dehydrogenase, a cytoplasmic enzyme, is not liberated during the reaction. The reaction requires the presence of Ca(2+), is enhanced by D(2)O and suppressed by colchicine. It is not, however, affected by dibutyryl cAMP or dibutyryl cGMP. No significant alteration of intracellular levels of cyclic AMP and cyclic GMP is observed during the incubation of mast cells with 0.1 mM H(2)O(2). These results indicate that microtubular functions would be involved in the releasing reaction although they are not under the control of cyclic nucleotides. Microscopic observation shows that H(2)O(2)-induced release is accompanied by degranulation.
用双氧水孵育大鼠腹膜肥大细胞会导致组胺大量释放。观察到 0.05-0.1mM H₂O₂时，释放量最大，但更高浓度的 H₂O₂反而会抑制释放。组胺释放始于约 2 分钟的滞后时间后，并在约 10 分钟达到平台期。如果孵育时间延长或在 10 分钟时给予额外的 H₂O₂，双氧水诱导的释放不会超过总组胺的 50-60%。这是因为 H₂O₂同时引起肥大细胞组胺释放系统的损伤和组胺的释放。双氧水诱导的释放不是由于细胞的非特异性溶解，因为细胞质酶乳酸脱氢酶在反应过程中不会释放。该反应需要 Ca²⁺的存在，D₂O 增强，秋水仙碱抑制。然而，它不受二丁酰环腺苷酸或二丁酰环鸟苷的影响。在 0.1mM H₂O₂孵育肥大细胞期间，细胞内 cAMP 和 cGMP 的水平没有观察到明显变化。这些结果表明，尽管微管功能不受环核苷酸的控制，但微管功能可能会参与释放反应。显微镜观察显示，H₂O₂诱导的释放伴随着脱颗粒。
