Department of Microbiology, Basic Medical Sciences Block, Panjab University, Chandigarh, India.
J Antimicrob Chemother. 2010 May;65(5):991-4. doi: 10.1093/jac/dkq066. Epub 2010 Mar 12.
Salmonella infections represent a major health hazard and have been responsible for a number of epidemics. In view of the emergence of multidrug-resistant Salmonella strains, there is a need for therapeutic alternatives. The purpose of this study was to evaluate the therapeutic potential of cryptdin-2 (a Paneth cell antimicrobial peptide) against Salmonella infection.
The bactericidal activity of cryptdin-2 against Salmonella enterica serovar Typhimurium NCTC74 was evaluated in vitro, ex vivo and in vivo on the basis of cfu enumeration.
The MBC of cryptdin-2 for Salmonella Typhimurium was found to be 19 mg/L. The ex vivo study demonstrated significantly higher intracellular killing of the bacteria by macrophages treated with cryptdin-2 as compared with untreated macrophages. Treatment of infected mice with cryptdin-2 resulted in significant clearance of Salmonella from livers, spleens and intestines.
The therapeutic efficacy of cryptdin-2 suggests that it may be a promising option to combat Salmonella infections or at least may act as an adjunct to conventional antibiotics.
沙门氏菌感染是一个主要的健康危害，已经导致了许多疫情的爆发。鉴于多药耐药性沙门氏菌菌株的出现，需要有治疗的替代方法。本研究旨在评估隐缩素-2（一种潘氏细胞抗菌肽）对沙门氏菌感染的治疗潜力。
根据 cfu 计数，体外、离体和体内评估隐缩素-2对肠炎沙门氏菌 NCTC74 的杀菌活性。
隐缩素-2对鼠伤寒沙门氏菌的 MBC 为 19 毫克/升。离体研究表明，用隐缩素-2处理的巨噬细胞对细菌的细胞内杀伤明显高于未处理的巨噬细胞。用隐缩素-2治疗感染的小鼠可显著清除肝脏、脾脏和肠道中的沙门氏菌。
隐缩素-2的治疗效果表明，它可能是一种有前途的对抗沙门氏菌感染的选择，或者至少可以作为传统抗生素的辅助手段。
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