Crop Research Institute, Drnovska 507, 16106 Praha 6, Ruzyne, Czech Republic.
Exp Appl Acarol. 2010 Oct;52(2):155-67. doi: 10.1007/s10493-010-9352-1. Epub 2010 Mar 13.
The study tested the effect of the chitin metabolic effectors, teflubenzuron, diflubenzuron, and calcofluor, and a combination of a chitinase and soybean trypsin inhibitor (STI) on the population growth of eight species of stored product mites under laboratory conditions. The compounds were incorporated into the diets of the mites in concentrations ranging from 0.01 to 50 mg g(-1). The final populations of mites were observed after 21 days of growth in controlled conditions. Diflubenzuron and calcofluor suppressed the growth of all the tested species more effectively than the other compounds. The doses required to suppress the mite populations to 50% (rc(50)) in comparison to the control ranged from 0.29 to 12.68 mg g(-1) for diflubenzuron and from 1.75 to 37.7 mg g(-1) for calcofluor, depending on the mite species. When tested at the highest concentration (10 mg g(-1)), teflubenzuron also suppressed all of the tested mite species in comparison to the control. The addition of chitinase/STI into the diet influenced population growth in several ways. When the highest concentration of chitinase in a cocktail of chitinase and STI (12.5 mg g(-1) of diet) was compared to the control, populations of Acarus siro, Aleuroglyphus ovatus and Aëroglyphus robustus decreased significantly, whereas populations of Tyroborus lini and Sancassania rodionovi increased significantly. The sensitivity of species to the tested compounds differed among species. The most tolerant species was S. rodionovi, the most sensitive was A. ovatus. The results confirmed that calcofluor and diflubenzuron have a toxic effect on stored product mites.
本研究测试了壳质代谢效应物（特氟苯隆、除虫脲和荧光增白剂）以及几丁质酶和大豆胰蛋白酶抑制剂（STI）混合物对实验室条件下 8 种储粮螨种群增长的影响。这些化合物以 0.01 至 50mg/g 的浓度添加到螨的饮食中。在控制条件下生长 21 天后观察到螨的最终种群。与其他化合物相比，除虫脲和荧光增白剂更有效地抑制了所有测试物种的生长。与对照相比，抑制螨种群到 50%（rc(50)）所需的剂量范围为 0.29 至 12.68mg/g 不等，具体取决于螨种；对于荧光增白剂，剂量范围为 1.75 至 37.7mg/g。当以最高浓度（10mg/g）测试时，与对照相比，特氟苯隆也抑制了所有测试的螨种。在饮食中添加几丁质酶/STI 以多种方式影响种群增长。与对照相比，当几丁质酶混合物中的几丁质酶最高浓度（饮食中 12.5mg/g）时，Acarus siro、Aleuroglyphus ovatus 和 Aëroglyphus robustus 的种群显著减少，而 Tyroborus lini 和 Sancassania rodionovi 的种群显著增加。物种对测试化合物的敏感性在物种间存在差异。最耐受的物种是 S. rodionovi，最敏感的物种是 A. ovatus。结果证实，荧光增白剂和除虫脲对储粮螨具有毒性作用。
