Cao X, Zhang W, Ma S, Zhang M, Wang J, Ye T
Department of Immunology, Second Military Medical University, 200433, Shanghai, China.
Sci China C Life Sci. 1997 Oct;40(5):539-45. doi: 10.1007/BF03183594.
To investigate the biological characterization and antitumor activities of GM-CSF gene-transfected dendritic cells, the splenic dendritic cells were infected with GM-CSF recombinant replication-deficient adenovirusesin vitro. Their enhanced expression of B7 was demonstrated by FACS analysis, and more potent stimulatory activity was confirmed by allogeneic MLR. Immunization of dendritic cells pulsed with irradiated B16 melanoma cells induced significant CTL and enabled host to resist the challenge of wild-type B16 cells. When they were transfected with GM-CSF gene subsequently, the induced CTL activity was higher, and the produced protection against B16 cell challenge and therapeutic effect on the mice with preestablished pulmonary metastases more effective. These data suggest that the dendritic cells pulsed with tumor antigen then transfected with GM-CSF gene can be used as an effective vaccine in tumor immunotherapy.
为研究GM-CSF基因转染的树突状细胞的生物学特性和抗肿瘤活性，体外将脾树突状细胞用GM-CSF重组复制缺陷型腺病毒感染。流式细胞术分析证实其B7表达增强，同种异体混合淋巴细胞反应证实其刺激活性更强。用经照射的B16黑色素瘤细胞脉冲处理的树突状细胞进行免疫可诱导显著的CTL，并使宿主抵抗野生型B16细胞的攻击。当随后用GM-CSF基因转染时，诱导的CTL活性更高，对B16细胞攻击产生的保护作用以及对已建立肺转移的小鼠的治疗效果更有效。这些数据表明，先用肿瘤抗原脉冲处理然后用GM-CSF基因转染的树突状细胞可作为肿瘤免疫治疗的有效疫苗。
